Anti-TRIB3 antibody (ab73547)
Key features and details
- Rabbit polyclonal to TRIB3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRIB3 antibody
See all TRIB3 primary antibodies -
Description
Rabbit polyclonal to TRIB3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide from the N-terminal domain of human TRIB3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TRIB3 antibody (ab73547)Anti-TRIB3 antibody (ab73547) at 1/500 dilution + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Additional bands at: 52 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
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Western blot - Anti-TRIB3 antibody (ab73547) Image courtesy of an anonymous Abreview.All lanes : Anti-TRIB3 antibody (ab73547) at 1/1000 dilution
Lanes 1-2 : Lysate prepared from rat soleus tissue, homogenized in TPER
Lane 3 : Lysate prepared from rat liver tissue, homogenized in TPER
Lysates/proteins at 60 µg per lane.
Secondary
All lanes : rabbit monoclonal conjugated to HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
