Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free (ab251152)
![Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free (ab251152)](https://www.abcam.com/ps/products/251/ab251152/Images/ab251152-407804-anti-pellino-1-pellino-2-antibody-epr19282-bsa-and-azide-free-western-blot.jpg "Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free (ab251152)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19282] to Pellino 1+Pellino 2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free
See all Pellino 1+Pellino 2 primary antibodies -
Description
Rabbit monoclonal [EPR19282] to Pellino 1+Pellino 2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251152 is the carrier-free version of ab192833. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251152 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR19282 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free (ab251152)All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/5000 dilution
Lane 1 : Human Pellino 1 full length recombinant protein
Lane 2 : Human Pellino 2 full length recombinant protein
Lane 3 : Human Pellino 3 full length recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDaThis data was developed using ab192833, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 1 second; Lanes 2-3: 1 minute.
Human Pellino 1 full length recombinant protein contains aa1-418 with a His-Tag®. Human Pellino 2 full length recombinant protein contains aa1-420 with a His-Tag®. Human Pellino 3 full length recombinant protein contains aa1-469 with a His-Tag®. All three recombinant proteins were made in-house.
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![Western blot - Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free (ab251152)](https://www.abcam.com/ps/products/251/ab251152/Images/ab251152-405636-anti-pellino-1-pellino-2-antibody-epr19282-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free (ab251152)All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/1000 dilution
Lane 1 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : Untreated RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 1 µg/ml LPS for 6 hours, whole cell lysate
Lane 4 : Untreated PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) treated with 1 µg/ml LPS for 6 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 3 minutesThis data was developed using ab192833, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The expression of Pellino 1 can be induced by LPS (PMID: 21204785).
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![Western blot - Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free (ab251152)](https://www.abcam.com/ps/products/251/ab251152/Images/ab251152-403317-anti-pellino-1-pellino-2-antibody-epr19282-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free (ab251152)All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 3 minutesThis data was developed using ab192833, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free (ab251152)](https://www.abcam.com/ps/products/251/ab251152/Images/ab251152-399144-anti-pellino-1-pellino-2-antibody-epr19282-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free (ab251152)All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 3 minutesThis data was developed using ab192833, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free (ab251152)](https://www.abcam.com/ps/products/251/ab251152/Images/ab251152-5-benefits-of-recombinant-antibodies.png)
Anti-Pellino 1+Pellino 2 antibody [EPR19282] - BSA and Azide free (ab251152)