Anti-S100A10 antibody [EPR3317] (ab76472)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3317] to S100A10
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Overview
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Product name
Anti-S100A10 antibody [EPR3317]
See all S100A10 primary antibodies -
Description
Rabbit monoclonal [EPR3317] to S100A10 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB MouseHuman -
Immunogen
Synthetic peptide within Human S100A10 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P60903 -
Positive control
- WB: HaCaT, A431, SW480, RAW264.7, NIH/3T3, and HT-29 cell lysates; IHC-P: Human squamous cell cervical carcinoma, colon tissue and human kidney tissue.
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General notes
The mouse recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse samples.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3317 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/10000 dilution (unpurified)
Lane 1 : HaCaT (Human skin keratinocyte) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 3 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa (possible non-specific binding)
Exposure time: 180 secondsBlocking buffer: 5% NFDM/TBST
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Immunohistochemical staining of paraffin embedded human squamous cell cervical carcinoma with purified ab76472 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Lane 1 : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/1000 dilution (purified)
Lane 2 : Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) at 1/5000 dilution
All lanes :Recombinant Human S100A10 protein (His tag) (ab221399)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa (possible dimer)
Exposure time: 3 secondsBlocking buffer: 5% NFDM/TBST
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All lanes : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/50000 dilution (purified)
Lane 1 : HACAT cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HT-29 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/5000 dilution (purified)
Lane 1 : RAW264.7 cell lysate
Lane 2 : NIH/3T3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Unpurified ab76472 at 1/100 dilution staining S100A10 in human colon tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Unpurified ab76472 at 1/100 dilution staining S100A10 in human kidney tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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