Human Annexin A10 ELISA Kit (ab222869)
Key features and details
- Sensitivity: 0.15 ng/ml
- Range: 0.156 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Annexin A10 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 1 20 3.6% Plasma 2 20 3.5% Plasma 3 20 3.9% Inter-assay Sample n Mean SD CV% Plasma 1 20 8.2% Plasma 2 20 8% Plasma 3 20 7.8% -
Sample type
Cell culture supernatant, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.15 ng/ml -
Range
0.156 ng/ml - 10 ng/ml -
Recovery
101 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat, Rabbit, Cow -
Product overview
ab222869 Human Annexin A10 ELISA Kit is designed for the quantitative measurement of Annexin A10 in plasma, serum, urine and cell culture samples.
The kit employs a quantitative sandwich enzyme immunoassay technique that measures human Annexin A10 in 4 hours. A polyclonal antibody specific for human Annexin A10 has been pre-coated onto a 96-well microplate with removable strips. Annexin A10 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Annexin A10, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Notes
Annexin A10, also known as ANXA10, Annexin-10, or Annexin-14, is a member of the annexin family of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and homologous C-terminal domains containing the calcium-dependent phospholipid-binding sites. Annexin A10 contains deduced 324 amino acids and weighs 37 kDa. It has several distinct features, including rare expression, a codon deletion in conserved repeat 3, and an unusual ablation of the type II calcium-binding sites in tetrad core repeats, indicating a calcium- and membrane-binding-independent function. Annexin A10 has been implicated in cellular functions in endocytosis and exocytosis, anticoagulant activity, cellular differentiation and proliferation, invasion, inhibition of calcium channels, and signal transduction pathways.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml Anti- Human Annexin A10 coated Microplate (12 x 8 wells) 1 unit Biotinylated Human Annexin A10 1 x 120µl Chromogen Substrate 1 x 8ml Human Annexin A10 Standard 1 vial Sealing Tapes 3 units Standard Diluent 1 x 2ml Stop Solution 1 x 12ml -
Research areas
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Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. - Information by UniProt
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Alternative names
- annexin 14
- Annexin A10
- Annexin-10
see all -
Database links
- Entrez Gene: 11199 Human
- Omim: 608008 Human
- SwissProt: Q9UJ72 Human
- Unigene: 188401 Human
Images
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Human Annexin A10 ELISA Kit (ab222869)
Example of human Annexin A10 standard curve.