Antibodies, Proteins, Kits and Reagents for Life Science

Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3317] to S100A10 - BSA and Azide free
Suitable for: IHC-P, WB
Reacts with: Mouse, Human

Product name

Anti-S100A10 antibody [EPR3317] - BSA and Azide free
See all S100A10 primary antibodies
Description

Rabbit monoclonal [EPR3317] to S100A10 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or ICC
Species reactivity

Reacts with: Mouse, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HaCaT, A431, SW480, RAW264.7, NIH/3T3, and HT-29 cell lysates; IHC-P: Human squamous cell cervical carcinoma, colon tissue and human kidney tissue.
General notes
ab232524 is the carrier-free version of ab76472 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab232524 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

The mouse recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse samples.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3317
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)

This data was developed using ab76472, the same antibody clone in a different buffer formulation.

Immunohistochemical staining of paraffin embedded human squamous cell cervical carcinoma with purified ab76472 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)

All lanes : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/10000 dilution (unpurified)

Lane 1 : HaCaT (Human skin keratinocyte) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 3 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 11 kDa
Observed band size: 13 kDa
why is the actual band size different from the predicted?
Additional bands at: 40 kDa (possible non-specific binding)

Exposure time: 180 seconds

Blocking buffer: 5% NFDM/TBST

This data was developed using ab76472, the same antibody clone in a different buffer formulation.
Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)

Lane 1 : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/1000 dilution (purified)
Lane 2 : Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) at 1/5000 dilution

All lanes : Recombinant Human S100A10 protein (His tag) (ab221399)

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 11 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa (possible dimer)

Exposure time: 3 seconds

Blocking buffer: 5% NFDM/TBST

This data was developed using ab76472, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)

This data was developed using ab76472, the same antibody clone in a different buffer formulation.

Unpurified ab76472 at 1/100 dilution staining S100A10 in human colon tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)

This data was developed using ab76472, the same antibody clone in a different buffer formulation.

Unpurified ab76472 at 1/100 dilution staining S100A10 in human kidney tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)

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