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Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3317] to S100A10 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-S100A10 antibody [EPR3317] - BSA and Azide free
    See all S100A10 primary antibodies

  • Description

    Rabbit monoclonal [EPR3317] to S100A10 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HaCaT, A431, SW480, RAW264.7, NIH/3T3, and HT-29 cell lysates; IHC-P: Human squamous cell cervical carcinoma, colon tissue and human kidney tissue.

  • General notes

    ab232524 is the carrier-free version of ab76472 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab232524 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    The mouse recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse samples.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)

    This data was developed using ab76472, the same antibody clone in a different buffer formulation.

    Immunohistochemical staining of paraffin embedded human squamous cell cervical carcinoma with purified ab76472 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
    Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
    All lanes : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/10000 dilution (unpurified)

    Lane 1 : HaCaT (Human skin keratinocyte) whole cell lysate
    Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
    Lane 3 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 4 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 11 kDa
    Observed band size: 13 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 40 kDa (possible non-specific binding)


    Exposure time: 180 seconds


    Blocking buffer: 5% NFDM/TBST

    This data was developed using ab76472, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
    Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
    Lane 1 : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/1000 dilution (purified)
    Lane 2 : Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) at 1/5000 dilution

    All lanes : Recombinant Human S100A10 protein (His tag) (ab221399)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 11 kDa
    Observed band size: 13 kDa why is the actual band size different from the predicted?
    Additional bands at: 25 kDa (possible dimer)


    Exposure time: 3 seconds


    Blocking buffer: 5% NFDM/TBST

    This data was developed using ab76472, the same antibody clone in a different buffer formulation.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)

    This data was developed using ab76472, the same antibody clone in a different buffer formulation.

    Unpurified ab76472 at 1/100 dilution staining S100A10 in human colon tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)

    This data was developed using ab76472, the same antibody clone in a different buffer formulation.

    Unpurified ab76472 at 1/100 dilution staining S100A10 in human kidney tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
    Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)

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