Anti-JNK1+JNK2 (phospho T183 + Y185) antibody (ab131499)
Key features and details
- Rabbit polyclonal to JNK1+JNK2 (phospho T183 + Y185)
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-JNK1+JNK2 (phospho T183 + Y185) antibody
See all JNK1+JNK2 primary antibodies -
Description
Rabbit polyclonal to JNK1+JNK2 (phospho T183 + Y185) -
Host species
Rabbit -
Specificity
The region of JNK1 and JNK2 surrounding T183 + Y185 has a high degree of similarity to the corresponding regions in JNK3 and thus may cross react with this protein if phosphorylated on the corresponding residues. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide from around the phosphorylation sites of threonine 183/tyrosine 185 (
M-M-T(p)-P-Y(p)- V - V
) of Human JNK1+JNK2 (NP_002741.1, NP_001128516.1, NP_002744.1)
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was purified by affinity chromatography using epitope-specific phosphopeptide. Non-phosphospecific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-JNK1+JNK2 (phospho T183 + Y185) antibody (ab131499)All lanes : Anti-JNK1+JNK2 (phospho T183 + Y185) antibody (ab131499) at 1/500 dilution
Lane 1 : C6 cells treated with anisomycin
Lane 2 : Untreated C6 cells
Predicted band size: 46, 54 kDaSecondary antibody - anti-rabbit HRP (ab6721)
-
Immunocytochemistry/ Immunofluorescence - Anti-JNK1+JNK2 (phospho T183 + Y185) antibody (ab131499)Immunofluorescence analysis of methanol fixed HeLa cells labelled with ab131499 at 1/100 dilution.
