HRP Anti-EAAT3 antibody [EPR6774(B)] (ab203479)
![HRP Anti-EAAT3 antibody [EPR6774(B)] (ab203479)](https://www.abcam.com/ps/products/203/ab203479/Images/ab203479-248500-anti-eaat3-antibody-epr6774b-hrp-western-blot.jpg "HRP Anti-EAAT3 antibody [EPR6774(B)] (ab203479)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR6774(B)] to EAAT3
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
-
Product name
HRP Anti-EAAT3 antibody [EPR6774(B)]
See all EAAT3 primary antibodies -
Description
HRP Rabbit monoclonal [EPR6774(B)] to EAAT3 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Fetal brain, Human testis and Fetal muscle tissue lysates.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Dissociation constant (KD)
KD = 3.80 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6774(B) -
Isotype
IgG -
Research areas
Images
-
Western blot - HRP Anti-EAAT3 antibody [EPR6774(B)] (ab203479)All lanes : HRP Anti-EAAT3 antibody [EPR6774(B)] (ab203479) at 1/5000 dilution
Lane 1 : Brain (Human) Tissue Lysate - fetal normal tissue
Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue
Lane 3 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 8 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab203479 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
-
![HRP Anti-EAAT3 antibody [EPR6774(B)] (ab203479)](https://www.abcam.com/ps/products/203/ab203479/Images/ab203479-3-benefits-of-recombinant-antibodies.png)
HRP Anti-EAAT3 antibody [EPR6774(B)] (ab203479)
