Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EAAT3 antibody [EPR6774(B)] (ab124802)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6774(B)] to EAAT3
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-EAAT3 antibody [EPR6774(B)]
    See all EAAT3 primary antibodies

  • Description

    Rabbit monoclonal [EPR6774(B)] to EAAT3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human EAAT3 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: Human fetal brain, human brain and human testis lysates . IHC-P: Human kidney tissue, and human glioma tissue. Flow Cyt: U-87 MG cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)
    Western blot - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)
    All lanes : Anti-EAAT3 antibody [EPR6774(B)] (ab124802) at 1/50000 dilution ((unpurified))

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : Human testis tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Observed band size: 70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking and Diluting buffer : 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue sections labeling EAAT3 with purified ab124802 at 1/1000 dilution (0.8 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)
    Western blot - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)
    Anti-EAAT3 antibody [EPR6774(B)] (ab124802) at 1/1000 dilution (Purified) + Human brain lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 70 kDa why is the actual band size different from the predicted?



    We are unsure how to define the extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)

    Unpurified ab124802, at 1/500 dilution, staining EAAT3 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)
    OI-RD Scanning - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-EAAT3 antibody [EPR6774(B)] (ab124802)
    Anti-EAAT3 antibody [EPR6774(B)] (ab124802)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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