Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MARK2 antibody [EPR8553] (ab133724)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8553] to MARK2
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-MARK2 antibody [EPR8553]
    See all MARK2 primary antibodies

  • Description

    Rabbit monoclonal [EPR8553] to MARK2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of Human MARK2 (Q7KZI7).

  • Positive control

    • Raji cells; RAW 264.7, NIH/3T3, Neuro-2a, Jurkat, Raji and U-87 MG cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)
    Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: MARK2 knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: Raw264.7 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab133724 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab133724 was shown to recognize MARK2 when MARK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and MARK2 knockout samples were subjected to SDS-PAGE. ab133724 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)  preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)
    Western blot - Anti-MARK2 antibody [EPR8553] (ab133724)
    All lanes : Anti-MARK2 antibody [EPR8553] (ab133724) at 1/1000 dilution

    Lane 1 : RAW 264.7 cell lysate
    Lane 2 : NIH/3T3 cell lysate
    Lane 3 : Neuro-2a cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : Raji cell lysate
    Lane 6 : U-87 MG cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 88 kDa

  • Flow Cytometry - Anti-MARK2 antibody [EPR8553] (ab133724)
    Flow Cytometry - Anti-MARK2 antibody [EPR8553] (ab133724)
    Flow cytometry analysis of permeabilized Raji cells staining MARK2, using ab133724 at a 1/100 dilution (red) or a control rabbit IgG antibody (green).
  • OI-RD Scanning - Anti-MARK2 antibody [EPR8553] (ab133724)
    OI-RD Scanning - Anti-MARK2 antibody [EPR8553] (ab133724)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-MARK2 antibody [EPR8553] (ab133724)
    Anti-MARK2 antibody [EPR8553] (ab133724)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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