Anti-MARK2 (phospho T595) antibody (ab34751)
Key features and details
- Rabbit polyclonal to MARK2 (phospho T595)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MARK2 (phospho T595) antibody
See all MARK2 primary antibodies -
Description
Rabbit polyclonal to MARK2 (phospho T595) -
Host species
Rabbit -
Specificity
Reactivity occurs against human MARK2 pT595 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human MARK2 is minimal by ELISA. Expect at least partial reactivity with other isoforms of MARK. The immunogen sequence is present in all forms identified to date and localizes to T595 on MARK2, T587 on MARK3, T591 on MARK1 and T568 on MARK4. -
Tested Applications & Species
See all applications and species dataApplication Species WB HumanRecombinant fragment
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Immunogen
Synthetic peptide corresponding to Human MARK2 aa 591-603.
Sequence:VSSRSTFHAGQLR
Database link: Q7KZI7 -
Positive control
- HEK293 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The product was affinity purified from monospecific antiserum by immunoaffinity purification. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MARK2 (phospho T595) antibody (ab34751)All lanes : Anti-MARK2 (phospho T595) antibody (ab34751) at 1/500 dilution
Lane 1 : MW markers
Lane 2 : untransfected HEK293 cell lysate
Lane 3 : HEK293 cells transfected with pCMV-3×Flag-
MARK2 plus pCMV-Flag-PKC-zeta
Lane 4 : HEK293 cells transfected with pCMV-3×Flag-MARK2 only
Predicted band size: 87 kDa
Observed band size: 82 kDa why is the actual band size different from the predicted?
