Anti-HOXD13 antibody (ab19866)
Key features and details
- Rabbit polyclonal to HOXD13
- Suitable for: WB, IHC-FoFr, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HOXD13 antibody
See all HOXD13 primary antibodies -
Description
Rabbit polyclonal to HOXD13 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-FoFr, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Xenopus laevis
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Immunogen
Synthetic peptide corresponding to Human HOXD13 aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab22466) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab19866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 36 kDa).IHC-FoFr 1/100 - 1/300.ICC/IF Use a concentration of 5 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 36 kDa).IHC-FoFr
1/100 - 1/300.ICC/IF
Use a concentration of 5 µg/ml.Target
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Relevance
This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on chromosome 2. Deletions that remove the entire HOXD gene cluster or the 5' end of this cluster have been associated with severe limb and genital abnormalities. Mutations in this particular gene cause synpolydactyly. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 396415 Chicken
- Entrez Gene: 3239 Human
- Entrez Gene: 15433 Mouse
- Entrez Gene: 288154 Rat
- Omim: 142989 Human
- SwissProt: P24344 Chicken
- SwissProt: P35453 Human
- SwissProt: P70217 Mouse
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Alternative names
- HOXD13 antibody
- BDE antibody
- BDSD antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HOXD13 antibody (ab19866)
ICC/IF image of ab19866 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab19866 at 5 ug/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-HOXD13 antibody (ab19866)This image is courtesy of Shunyou Wang, Ph.D.ab19866 was tested in IHC of formalin fixed sections from mouse (over two weeks of age) lung. Antigen retrieval was in citrate buffer and blocking was in 1% BSA. The primary antibody was incubated at a dilution of 1/100 overnight at 4 degrees celsius. The secondary antibody was a biotin conjugated anti-rabbit IgG. -
Western blot - Anti-HOXD13 antibody (ab19866)Anti-HOXD13 antibody (ab19866) at 1 µg/ml + HOXD13 (Human) Recombinant Protein at 0.2 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?ab19866 was tested on a recombinant GST tagged HOXD13 protein (Human) predicted to run at 65 kDa.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab19866 has been referenced in 10 publications.
- Bolt CC et al. Context-dependent enhancer function revealed by targeted inter-TAD relocation. Nat Commun 13:3488 (2022). PubMed: 35715427
- Bolt CC et al. Mesomelic dysplasias associated with the HOXD locus are caused by regulatory reallocations. Nat Commun 12:5013 (2021). PubMed: 34408147
- Di Mauro A et al. Aberrant Expression of Long Non Coding RNA HOTAIR and De-Regulation of the Paralogous 13 HOX Genes Are Strongly Associated with Aggressive Behavior of Gastro-Entero-Pancreatic Neuroendocrine Tumors. Int J Mol Sci 22:N/A (2021). PubMed: 34208964
- Basu S et al. Unblending of Transcriptional Condensates in Human Repeat Expansion Disease. Cell 181:1062-1079.e30 (2020). PubMed: 32386547
- Tatangelo F et al. Posterior HOX genes and HOTAIR expression in the proximal and distal colon cancer pathogenesis. J Transl Med 16:350 (2018). PubMed: 30541551
- Aquino G et al. Deregulation of paralogous 13 HOX genes in oral squamous cell carcinoma. Am J Cancer Res 5:3042-55 (2015). PubMed: 26693058
- Cantile M et al. Aberrant expression of posterior HOX genes in well differentiated histotypes of thyroid cancers. Int J Mol Sci 14:21727-40 (2013). IHC-P ; Human . PubMed: 24189220
- Illig R et al. Spatio-temporal expression of HOX genes in human hindgut development. Dev Dyn 242:53-66 (2013). PubMed: 23073994
- Illig R et al. Breaking the seals: efficient mRNA detection from human archival paraffin-embedded tissue. RNA 15:1588-96 (2009). IHC-P ; Human . PubMed: 19549718
- Cantile M et al. HOX D13 expression across 79 tumor tissue types. Int J Cancer 125:1532-41 (2009). PubMed: 19488988
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HOXD13 antibody (ab19866)
ICC/IF image of ab19866 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab19866 at 5 ug/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-HOXD13 antibody (ab19866) This image is courtesy of Shunyou Wang, Ph.D.ab19866 was tested in IHC of formalin fixed sections from mouse (over two weeks of age) lung. Antigen retrieval was in citrate buffer and blocking was in 1% BSA. The primary antibody was incubated at a dilution of 1/100 overnight at 4 degrees celsius. The secondary antibody was a biotin conjugated anti-rabbit IgG.
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Western blot - Anti-HOXD13 antibody (ab19866)Anti-HOXD13 antibody (ab19866) at 1 µg/ml + HOXD13 (Human) Recombinant Protein at 0.2 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?ab19866 was tested on a recombinant GST tagged HOXD13 protein (Human) predicted to run at 65 kDa.
