Anti-GCSF Receptor antibody (ab126167)
Key features and details
- Rabbit polyclonal to GCSF Receptor
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GCSF Receptor antibody
See all GCSF Receptor primary antibodies -
Description
Rabbit polyclonal to GCSF Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within internal sequence amino acids 164-392 of Human GCSF Receptor (UniProt Q99062).
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Positive control
- IHC-P: Human breast carcinoma tissue. IF: THP-1 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCSF Receptor antibody (ab126167)
Formalin-fixed, paraffin embedded human breast carcinoma tissue stained fro GCSF receptor usinf a126167 at 1/250 dilution in immunohistochemical analysis,
Antigen Retrieval: EDTA based, pH 8.0 buffer, 15min
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Immunocytochemistry/ Immunofluorescence - Anti-GCSF Receptor antibody (ab126167)Immunofluorescence analysis of THP-1 (human monocytic leukemia cell line) cells labeling GCSF (green) using ab126167 at 1/500 dilution. Blue: Hoechst 33342 staining. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
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Western blot - Anti-GCSF Receptor antibody (ab126167)All lanes : Anti-GCSF Receptor antibody (ab126167) at 1/500 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 92 kDa7.5% SDS-PAGE
