Antibodies, Proteins, Kits and Reagents for Life Science

300 865 ₸ Product size

100 µl 300 865 ₸

Order now and get it on Tuesday March 09, 2021

Anti-GDF15 antibody [EPR19939] (ab206414)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19939] to GDF15
Suitable for: IHC-P, WB, IP, ELISA
Reacts with: Human

Product name

Anti-GDF15 antibody [EPR19939]
See all GDF15 primary antibodies
Description

Rabbit monoclonal [EPR19939] to GDF15
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HepG2 and LNCaP whole cell lysates; human prostate cancer and placenta lysates; untreated HT1080 and treated with 200ng/ml Phorbol-12-myristate-13-acetate (TPA) for 24 hours whole cell lysates. IHC-P: Human placenta and prostate hyperplasia tissues. IP: HepG2 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19939
Isotype

IgG
Research areas

Western blot - Anti-GDF15 antibody [EPR19939] (ab206414)

All lanes : Anti-GDF15 antibody [EPR19939] (ab206414) at 1/1000 dilution

Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : LNCaP (Human prostate cancer cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 34 kDa
Observed band size: 12.5,35 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 3 minutes; Lane 2: 10 seconds.

The molecular weight observed is consistent with the literature (PMID: 20884666).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF15 antibody [EPR19939] (ab206414)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling GDF15 with ab206414 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on human placenta is observed (PMID: 9593718). Positive staining on human placenta and prostate hyperplasia, other tissues tested were negative.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-GDF15 antibody [EPR19939] (ab206414)

All lanes : Anti-GDF15 antibody [EPR19939] (ab206414) at 1/1000 dilution

Lane 1 : Human prostate cancer lysate
Lane 2 : Human placenta lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 34 kDa
Observed band size: 12.5 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 10 seconds; Lane 2: 3 minutes.

The molecular weight observed is consistent with the literature (PMID: 20884666).
Western blot - Anti-GDF15 antibody [EPR19939] (ab206414)

All lanes : Anti-GDF15 antibody [EPR19939] (ab206414) at 1/1000 dilution

Lane 1 : Untreated HT1080 (Human fibrosarcoma cell line) whole cell lysate
Lane 2 : HT1080 (Human fibrosarcoma cell line) whole cell lysate treated with 200ng/ml Phorbol-12-myristate-13-acetate (TPA) for 24 hours

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression level of GDF15 can be increased by TPA treatment (PMID: 15757899).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF15 antibody [EPR19939] (ab206414)

Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue labeling GDF15 with ab206414 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on human prostate hyperplasia is observed (PMID: 9593718). Positive staining on human placenta and prostate hyperplasia, other tissues tested were negative.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-GDF15 antibody [EPR19939] (ab206414)

GDF15 was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab206414 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab206414 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: HepG2 whole cell lysate 10µg (Input).

Lane 2: ab206414 IP in HepG2 whole cell lysate.

Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab206414 in HepG2 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
ELISA - Anti-GDF15 antibody [EPR19939] (ab206414)

ELISA analysis of ab206414 binding functionally active mature GDF15 (ab50077) at 1 µg/mL.
Western blot - Anti-GDF15 antibody [EPR19939] (ab206414)

All lanes : Anti-GDF15 antibody [EPR19939] (ab206414) at 1/1000 dilution

Lane 1 : Untreated HT1080 (Human fibrosarcoma cell line) whole cell lysate
Lane 2 : HT1080 (Human fibrosarcoma cell line) whole cell lysate treated with 200ng/ml Phorbol-12-myristate-13-acetate (TPA) for 24 hours

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.

Both pro and mature form can be increased by TPA treatment.
Anti-GDF15 antibody [EPR19939] (ab206414)

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