Anti-GDF15 antibody (ab39999)
Key features and details
- Goat polyclonal to GDF15
- Suitable for: ICC, WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GDF15 antibody
See all GDF15 primary antibodies -
Description
Goat polyclonal to GDF15 -
Host species
Goat -
Tested applications
Suitable for: ICC, WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide: QKTDTGVSLQTYDD, corresponding to C terminal amino acids 286-299 of Human GDF15
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GDF15 antibody (ab39999)All lanes : Anti-GDF15 antibody (ab39999) at 0.3 µg/ml
Lane 1 : Human Testes whole cell lysate in RIPA buffer
Lane 2 : Human Skin whole cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 34 kDa
Observed band size: 30-35 kDa why is the actual band size different from the predicted?
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Immunocytochemistry - Anti-GDF15 antibody (ab39999) This image is courtesy of an abreview submitted by Dr. Ruma Raha-Chowdhury, Cambridge University, United Kingdom.ICC/IF image of human bone marrow smear stained with Ab39999(1/250). The smear was permeabilized in 0.1% PBS-Triton X for 1h and incubated with 10% donkey serum for 1hr at 24°C to block non-specific protein-protein interactions. The sections were then incubated with ab39999 used at 1/250 dilution, overnight at +4°C. The secondary antibody was Alexa Fluor® 568 donkey anti-goat used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Flow Cytometry - Anti-GDF15 antibody (ab39999)Flow cytometric analysis of paraformaldehyde fixed, 0.5% Triton permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling GDF-15 using ab39999 at 10 μg/ml followed by Alexa Fluor 488 secondary antibody (blue) at 1 μg/ml. Unimmunized goat IgG was used as isotype control, followed by Alexa Fluor 488 secondary antibody (black).
