Mouse IFN beta ELISA Kit (ab252363)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 28 pg/ml
- Range: 39.1 pg/ml - 2500 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IFN beta ELISA Kit
See all Interferon beta kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 4.9% Inter-assay Sample n Mean SD CV% Supernatant 3 3.6% -
Sample type
Cell culture supernatant, Serum, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
28 pg/ml -
Range
39.1 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 97 94% - 100% Serum 86 84% - 87% Cell culture media 88 78% - 98% Cit plasma 84 81% - 87% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse IFN beta ELISA Kit (ab252363) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IFN beta protein in cell culture supernatant, cit plasma, serum, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IFN beta with 28 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse IFN beta (Interferon beta 1) Capture Antibody 1 x 600µl 10X Mouse IFN beta (Interferon beta 1) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse IFN beta (Interferon beta 1) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Has antiviral, antibacterial and anticancer activities. -
Sequence similarities
Belongs to the alpha/beta interferon family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- beta-interferon
- Fibroblast interferon
- IFB
see all -
Database links
- Entrez Gene: 15977 Mouse
- SwissProt: P01575 Mouse
- Unigene: 1245 Mouse
Images
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Other - Mouse IFN beta ELISA Kit (ab252363)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse IFN beta standard curve in Sample Diluent NS.The IFN beta standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IFN beta in LPS stimulated Raw264.7 cell culture supernatant samples.The concentrations of IFN beta were measured in duplicates, interpolated from the IFN beta standard curves and corrected for sample dilution. Undiluted samples are as follows: Raw264.7 cell culture supernatant 25%, The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFN beta concentration was determined to be 6,664 pg/mL in Raw264.7 cell culture supernatant. Mouse IFN beta was below the detection point in unstimulated Raw264.7 supernatant.
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Sandwich ELISA - Mouse IFN beta ELISA Kit (ab252363)To learn more about the advantages of recombinant antibodies see here.
