Human POR (Cytochrome P450 Reductase) knockout HCT116 cell lysate (ab257596)
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab263569 - Human POR knockout HCT116 cell lysate 1 x 100µg ab255555 - Human wild-type HCT116 cell lysate 1 x 100µg -
Research areas
-
Cell type
epithelial -
Disease
Carcinoma -
STR Analysis
Amelogenin X D5S818: 10, 11 D13S317: 10, 12 D7S820: 11, 12 D16S539: 11, 13 vWA: 17, 22 TH01: 8,9 TPOX: 8, 9 CSF1PO: 7, 10
Images
-
Western blot - Human POR (Cytochrome P450 Reductase) knockout HCT116 cell lysate (ab257596)Lane 1: Wild-type HCT116 cell lysate (40µg)
Lane 2: POR knockout HCT116 cell lysate (40µg)
Lane 3: HepG2 cell lysate (40µg)
Lane 4: A431 cell lysate (40µg)
Lanes 1- 4: Merged signal (red and green). Green - ab180597 observed at 80 kDa. Red - loading control ab8245 observed at 37 kDa.
ab180597 Rabbit monoclonal [EPR14479(B)] to Cytochrome P450 Reductase was shown to specifically react with Cytochrome P450 Reductase in wild-type HCT116 cells in western blot. Loss of signal was observed when knockout cell line ab266889 (knockout cell lysate ab257596) was used. Wild-type and Cytochrome P450 Reductase knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab180597 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human POR knockout HCT116 cell lysate (ab257596)Homozygous: Insertion of the selection cassette in exon4
