Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GDF15 antibody [7C12.B3.F2] (ab106112)

Key features and details

  • Mouse monoclonal [7C12.B3.F2] to GDF15
  • Suitable for: ICC, WB
  • Reacts with: Human, Recombinant fragment
  • Isotype: unknown

Overview

  • Product name

    Anti-GDF15 antibody [7C12.B3.F2]
    See all GDF15 primary antibodies

  • Description

    Mouse monoclonal [7C12.B3.F2] to GDF15

  • Host species

    Mouse

  • Specificity

    ab106112 specifically reacts with an H variant sequence of GDF15 protein.

  • Tested applications

    Suitable for: ICC, WBmore details

  • Species reactivity

    Reacts with: Human, Recombinant fragment
    Predicted to work with: Chimpanzee, Macaque monkey

  • Immunogen

    A synthetic peptide corresponding to a region near the amino terminal end of Human GDF15 protein. A residue of cysteine was added to facilitate coupling to KLH.

  • Positive control

    • Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant with SUMO expression tag.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)
    Immunocytochemistry - Anti-GDF15 antibody [7C12.B3.F2] (ab106112) This image is courtesy of Dr. Ruma Raha-Chowdhury (Cambridge University, United Kingdom)

    ICC/IF image of GDF15 staining on human blood film using ab106112 (1:1000). The cells were fixed using formaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. ab106112 was diluted to 1:1000 and incubated with cells for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to mouse IgG conjugated to Alexa Fluor 568.

  • Western blot - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)
    Western blot - Anti-GDF15 antibody [7C12.B3.F2] (ab106112)
    All lanes : Anti-GDF15 antibody [7C12.B3.F2] (ab106112) at 1/1000 dilution

    Lane 1 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant with SUMO expression tag
    Lane 2 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 D variant with SUMO expression tag
    Lane 3 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 H variant
    Lane 4 : Pichia pastoris (yeast) whole cell lysate expressing recombinant GDF15 D variant

    Secondary
    All lanes : Peroxidase conjugated Gt-a-Mouse IgG at 1/40000 dilution

    Performed under reducing conditions.

    Predicted band size: 34 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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