Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H1 (tri methyl K25) antibody (ab17347)

Key features and details

  • Rabbit polyclonal to Histone H1 (tri methyl K25)
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Histone H1 (tri methyl K25) antibody

  • Description

    Rabbit polyclonal to Histone H1 (tri methyl K25)

  • Host species

    Rabbit

  • Specificity

    ab17347 gives a positive result against the immunizing peptide and a negative result against the corresponding unmodified peptide in ELISA analysis. ab17347 recognises histone H1 tri methyl K25 in HeLa nuclear and whole cell lysates at 35 kDa. The signal is efficiently blocked using the immunzing histone H1 tri methyl K25 peptide but not the corrseponding unmodified peptide or histone H1 di methyl K25 peptide. ab17347 also appears to recognise other bands between 34 kDa and 37 kDa, which are attributed to the antibody recognising other isoforms of histone H1. ab17347 also appears to recognise methylated histone H3 at 17 kDa.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Histone H1 aa 1-100 (internal sequence) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab17587)

  • General notes

    HeLa nuclear lysate

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab17347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Cow
Non human primates
Application Abreviews Notes
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
ICC/IF (1)
Use a concentration of 1 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
ICC/IF
Use a concentration of 1 µg/ml.

Target

  • Relevance

    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in the formation of higher order structure in chromatin and the maintenance of overall chromatin compaction. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono-, di-, or trimethylated state. Arginine resdiues can also by mono or di methylated.

  • Cellular localization

    Nuclear

  • Database links

    • Alternative names

      • H1 antibody
      • H1.4 antibody
      • histone H1.4 antibody

    Images

    • Western blot - Anti-Histone H1 (tri methyl K25) antibody (ab17347)
      Western blot - Anti-Histone H1 (tri methyl K25) antibody (ab17347)
      All lanes : Anti-Histone H1 (tri methyl K25) antibody (ab17347) at 1 µg/ml

      Lane 1 : HeLa nuclear lysate
      Lane 2 : HeLa whole cell lysate
      Lane 3 : HeLa nuclear lysate with Human Histone H1 (tri methyl K25) peptide (ab17587) at 1 µg/ml
      Lane 4 : HeLa whole cell lysate with Human Histone H1 (tri methyl K25) peptide (ab17587) at 1 µg/ml
      Lane 5 : HeLa nuclear lysate with Human Histone H1 peptide (ab17588) at 1 µg/ml
      Lane 6 : HeLa whole cell lysate with Human Histone H1 peptide (ab17588) at 1 µg/ml
      Lane 7 : HeLa nuclear lysate with Human Histone H1 (di methyl K25) peptide (ab21998) at 1 µg/ml
      Lane 8 : HeLa whole cell lysate with Human Histone H1 (di methyl K25) peptide (ab21998) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution at 1/10000 dilution

      Predicted band size: 35 kDa
      Observed band size: 35 kDa
      Additional bands at: 17 kDa (possible cross reactivity)



      ab17347 recognises histone H1 tri methyl K25 in HeLa nuclear (lane1) and whole cell (lane2) lysates at 35 kDa. The signal is efficiently blocked using the immunizing histone H1 tri methyl K25 peptide (lanes3-4) but not the corrseponding unmodified peptide (lanes5-6) or histone H1 di methyl K25 peptide (lanes7-8).

      ab17347 also appears to recognise other bands between 34 kDa and 37 kDa, which are attributed to the antibody recognising other isoforms of histone H1.

      The strong signal at 17 kDa is attributed methylated histone H3.
    • Immunocytochemistry/ Immunofluorescence - Anti-Histone H1 (tri methyl K25) antibody (ab17347)
      Immunocytochemistry/ Immunofluorescence - Anti-Histone H1 (tri methyl K25) antibody (ab17347)

      ICC/IF image of ab17347 stained human HepG2 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab17347, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293, MCF7 cells.

    References (1)

    Publishing research using ab17347? Please let us know so that we can cite the reference in this datasheet.

    ab17347 has been referenced in 1 publication.

    • Agger K  et al. UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene regulation and development. Nature 449:731-4 (2007). ICC/IF ; Human . PubMed: 17713478

    Images

    • Western blot - Anti-Histone H1 (tri methyl K25) antibody (ab17347)
      Western blot - Anti-Histone H1 (tri methyl K25) antibody (ab17347)
      All lanes : Anti-Histone H1 (tri methyl K25) antibody (ab17347) at 1 µg/ml

      Lane 1 : HeLa nuclear lysate
      Lane 2 : HeLa whole cell lysate
      Lane 3 : HeLa nuclear lysate with Human Histone H1 (tri methyl K25) peptide (ab17587) at 1 µg/ml
      Lane 4 : HeLa whole cell lysate with Human Histone H1 (tri methyl K25) peptide (ab17587) at 1 µg/ml
      Lane 5 : HeLa nuclear lysate with Human Histone H1 peptide (ab17588) at 1 µg/ml
      Lane 6 : HeLa whole cell lysate with Human Histone H1 peptide (ab17588) at 1 µg/ml
      Lane 7 : HeLa nuclear lysate with Human Histone H1 (di methyl K25) peptide (ab21998) at 1 µg/ml
      Lane 8 : HeLa whole cell lysate with Human Histone H1 (di methyl K25) peptide (ab21998) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution at 1/10000 dilution

      Predicted band size: 35 kDa
      Observed band size: 35 kDa
      Additional bands at: 17 kDa (possible cross reactivity)



      ab17347 recognises histone H1 tri methyl K25 in HeLa nuclear (lane1) and whole cell (lane2) lysates at 35 kDa. The signal is efficiently blocked using the immunizing histone H1 tri methyl K25 peptide (lanes3-4) but not the corrseponding unmodified peptide (lanes5-6) or histone H1 di methyl K25 peptide (lanes7-8).

      ab17347 also appears to recognise other bands between 34 kDa and 37 kDa, which are attributed to the antibody recognising other isoforms of histone H1.

      The strong signal at 17 kDa is attributed methylated histone H3.
    • Immunocytochemistry/ Immunofluorescence - Anti-Histone H1 (tri methyl K25) antibody (ab17347)
      Immunocytochemistry/ Immunofluorescence - Anti-Histone H1 (tri methyl K25) antibody (ab17347)

      ICC/IF image of ab17347 stained human HepG2 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab17347, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293, MCF7 cells.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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