Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ALDH2 antibody [EPR4494] (ab133306)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4494] to ALDH2
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ALDH2 antibody [EPR4494]
    See all ALDH2 primary antibodies

  • Description

    Rabbit monoclonal [EPR4494] to ALDH2

  • Host species

    Rabbit

  • Specificity

    The antibody immunogen has 87% homology with ALDH1A2 (O94788) and ALDH1A1 (P00352). Therefore a cross-reactivity with these proteins is likely.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues in Human ALDH2 (P05091).

  • Positive control

    • WB: SW480 and HepG2 cell lysates; Human heart, fetal liver, mouse liver, rat liver, and mouse lung lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ALDH2 antibody [EPR4494] (ab133306)
    Western blot - Anti-ALDH2 antibody [EPR4494] (ab133306)
    All lanes : Anti-ALDH2 antibody [EPR4494] (ab133306) at 1/1000 dilution

    Lane 1 : Wild-type SW480 cell lysate
    Lane 2 : ALDH2 knockout SW480 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 53 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab133306 observed at 53 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab133306 was shown to react with ALDH2 in wild-type SW480 cells in western blot. Loss of signal was observed when ALDH2 knockout sample was used. Wild-type and ALDH2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk before incubation with ab133306 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ALDH2 antibody [EPR4494] (ab133306)
    Western blot - Anti-ALDH2 antibody [EPR4494] (ab133306)
    All lanes : Anti-ALDH2 antibody [EPR4494] (ab133306) at 1/1000 dilution

    Lane 1 : HepG2 lysates
    Lane 2 : Human heart lysates
    Lane 3 : Fetal liver lysates
    Lane 4 : Mouse liver lysates
    Lane 5 : Rat liver lysates
    Lane 6 : Mouse lung lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 56 kDa

  • Anti-ALDH2 antibody [EPR4494] (ab133306)
    Anti-ALDH2 antibody [EPR4494] (ab133306)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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