Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 09, 2021

Anti-ALDH2 antibody [EPR4494] (ab133306)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4494] to ALDH2
Suitable for: WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-ALDH2 antibody [EPR4494]
See all ALDH2 primary antibodies
Description

Rabbit monoclonal [EPR4494] to ALDH2
Host species

Rabbit
Specificity

The antibody immunogen has 87% homology with ALDH1A2 (O94788) and ALDH1A1 (P00352). Therefore a cross-reactivity with these proteins is likely.
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to residues in Human ALDH2 (P05091).
Positive control
- WB: SW480 and HepG2 cell lysates; Human heart, fetal liver, mouse liver, rat liver, and mouse lung lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4494
Isotype

IgG
Research areas

Western blot - Anti-ALDH2 antibody [EPR4494] (ab133306)

All lanes : Anti-ALDH2 antibody [EPR4494] (ab133306) at 1/1000 dilution

Lane 1 : Wild-type SW480 cell lysate
Lane 2 : ALDH2 knockout SW480 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : MCF7 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 56 kDa
Observed band size: 53 kDa
why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab133306 observed at 53 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab133306 was shown to react with ALDH2 in wild-type SW480 cells in western blot. Loss of signal was observed when ALDH2 knockout sample was used. Wild-type and ALDH2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk before incubation with ab133306 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-ALDH2 antibody [EPR4494] (ab133306)

All lanes : Anti-ALDH2 antibody [EPR4494] (ab133306) at 1/1000 dilution

Lane 1 : HepG2 lysates
Lane 2 : Human heart lysates
Lane 3 : Fetal liver lysates
Lane 4 : Mouse liver lysates
Lane 5 : Rat liver lysates
Lane 6 : Mouse lung lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 56 kDa
Anti-ALDH2 antibody [EPR4494] (ab133306)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com