Anti-FADD (phospho S194) antibody (ab55334)
Key features and details
- Rabbit polyclonal to FADD (phospho S194)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FADD (phospho S194) antibody
See all FADD primary antibodies -
Description
Rabbit polyclonal to FADD (phospho S194) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of FADD only when phosphorylated at serine 194. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phosphopeptide derived from human FADD around the phosphorylation site of serine 194 (A-M-SP-P-M).
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Positive control
- Extracts from HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FADD (phospho S194) antibody (ab55334)All lanes : Anti-FADD (phospho S194) antibody (ab55334) at 1/500 dilution
Lane 1 : Extracts from HeLa cells treated
with Paclitaxel (1µM, 60mins).
Lane 2 : Extracts from HeLa cells treated
with Paclitaxel (1µM, 60mins), and with the immunising phosphpeptide.
Predicted band size: 23 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
