Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FADD antibody [EPR4415] - BSA and Azide free (ab232045)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4415] to FADD - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, ICC, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-FADD antibody [EPR4415] - BSA and Azide free
    See all FADD primary antibodies

  • Description

    Rabbit monoclonal [EPR4415] to FADD - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human FADD aa 1-150. The exact sequence is proprietary.
    Database link: Q13158

  • Positive control

    • WB: A431, Jurkat, HeLa and HAP1 cell lysates. Flow Cyt: A431 cells. IHC-P: Human kidney tissue.

  • General notes

    ab232045 is the carrier-free version of ab108601 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab232045 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-FADD antibody [EPR4415] - BSA and Azide free (ab232045)
    Western blot - Anti-FADD antibody [EPR4415] - BSA and Azide free (ab232045)
    All lanes : Anti-FADD antibody [EPR4415] (ab108601) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : FADD knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab108601).

    Lanes 1 - 2: Merged signal (red and green). Green - ab108601 observed at 23 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab108601 was shown to react with FADD in wild-type HeLa cells in western blot with loss of signal observed in FADD knockout cell line ab261817 (FADD knockout cell lysate ab257261). Wild-type and FADD knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108601 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-FADD antibody [EPR4415] - BSA and Azide free (ab232045)
    Flow Cytometry - Anti-FADD antibody [EPR4415] - BSA and Azide free (ab232045)

    Flow Cytometry analysis of A431(human epidermoid carcinoma) cells labeling FADD with purified ab108601 at 1/140 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108601).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR4415] - BSA and Azide free (ab232045)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR4415] - BSA and Azide free (ab232045)

    Immunohistochemical staining of paraffin-embedded Human kidney tissue using ab108601 at a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108601).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-FADD antibody [EPR4415] - BSA and Azide free (ab232045)
    Western blot - Anti-FADD antibody [EPR4415] - BSA and Azide free (ab232045)
    All lanes : Anti-FADD antibody [EPR4415] (ab108601) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : FADD knockout HAP1 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 23 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab108601 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab108601 was shown to specifically react with FADD when FADD knockout samples were used. Wild-type and FADD knockout samples were subjected to SDS-PAGE. ab108601 and ab8245 (loading control to GAPDH) were diluted 1/1,000 and 1/2,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108601).

  • Anti-FADD antibody [EPR4415] - BSA and Azide free (ab232045)
    Anti-FADD antibody [EPR4415] - BSA and Azide free (ab232045)

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