Anti-FADD antibody [OTI1C11] (ab119059)
![Anti-FADD antibody [OTI1C11] (ab119059)](https://www.abcam.com/ps/products/119/ab119059/Images/ab119059-389703-anti-fadd-antibody-oti1c11-western-blot-human-lysate.jpg "Anti-FADD antibody [OTI1C11] (ab119059)")
Key features and details
- Mouse monoclonal [OTI1C11] to FADD
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-FADD antibody [OTI1C11]
See all FADD primary antibodies -
Description
Mouse monoclonal [OTI1C11] to FADD -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human FADD. Protein produced in HEK293T cells
Database link: NP_003815 -
Positive control
- WB: HEK-293T cells transfected with pCMV6-ENTRY FADD cDNA, A431, HepG2, HeLa, A549, MCF7 and Jurkat cell lysates. Flow Cyt: HEK-293T cells transfected with a FADD overexpression plasmid.
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General notes
The clone number has been updated from 1C11 to OTI1C11, both clone numbers name the same clone.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography -
Clonality
Monoclonal -
Clone number
OTI1C11 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-FADD antibody [OTI1C11] (ab119059)All lanes : Anti-FADD antibody [OTI1C11] (ab119059) at 1/2000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : FADD knockout HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 23 kDaLanes 1-4: Merged signal (red and green). Green - ab119059 observed at 25 kDa. Red - loading control ab181602 observed at 37 kDa.
ab119059 Anti-FADD antibody [OTI1C11] was shown to specifically react with FADD in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261817 (knockout cell lysate ab257261) was used. Wild-type and FADD knockout samples were subjected to SDS-PAGE. ab119059 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-FADD antibody [OTI1C11] (ab119059)](https://www.abcam.com/ps/products/119/ab119059/Images/ab119059-256327-anti-fadd-antibody-oti1c11-western-blot.jpg)
Western blot - Anti-FADD antibody [OTI1C11] (ab119059)All lanes : Anti-FADD antibody [OTI1C11] (ab119059) at 1/2000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : FADD knockout HAP1 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 23 kDaLanes 1 - 4: Merged signal (red and green). Green - ab119059 observed at 25 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab119059 was shown to specifically react with FADD when FADD knockout samples were used. Wild-type and FADD knockout samples were subjected to SDS-PAGE. ab119059 and ab181602 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-FADD antibody [OTI1C11] (ab119059)](https://www.abcam.com/ps/products/119/ab119059/Images/ab119059-131877-anti-fadd-antibody-oti1c11-western-blot.jpg)
Western blot - Anti-FADD antibody [OTI1C11] (ab119059)All lanes : Anti-FADD antibody [OTI1C11] (ab119059) at 1/2000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY FADD cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 23 kDa
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![Western blot - Anti-FADD antibody [OTI1C11] (ab119059)](https://www.abcam.com/ps/products/119/ab119059/Images/ab119059-314231-anti-fadd-antibody-oti1c11-western-blot.jpg)
Western blot - Anti-FADD antibody [OTI1C11] (ab119059)All lanes : Anti-FADD antibody [OTI1C11] (ab119059) at 1/200 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa
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![Flow Cytometry - Anti-FADD antibody [OTI1C11] (ab119059)](https://www.abcam.com/ps/products/119/ab119059/Images/ab119059-131876-anti-fadd-antibody-oti1c11-flow-cytometry.jpg)
Flow Cytometry - Anti-FADD antibody [OTI1C11] (ab119059)HEK293T cells transfected with either a FADD overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained using ab119059 at 1/100 dilution and then analyzed by flow cytometry.
