Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 103 862 ₸

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Anti-TL1A antibody [EPR23330-2] (ab270456)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23330-2] to TL1A
Suitable for: WB, IP
Reacts with: Human

Product name

Anti-TL1A antibody [EPR23330-2]
See all TL1A primary antibodies
Description

Rabbit monoclonal [EPR23330-2] to TL1A
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HUVEC, HUVEC (treated with/ml TNF-alpha for 24 hours) and HT-29 whole cell lysates. IP: HT-29 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23330-2
Isotype

IgG
Research areas

Western blot - Anti-TL1A antibody [EPR23330-2] (ab270456)

All lanes : Anti-TL1A antibody [EPR23330-2] (ab270456) at 1/1000 dilution

Lane 1 : HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 28 kDa
Observed band size: 25-36 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.

TL1A is a glycoprotein, the molecular weight observed is consistent with what has been described in literature (PMID 20675618).

Negative control: MCF7 (PMID: 9434163 ).

This blot was developed using a higher sensitivity ECL substrate.
Immunoprecipitation - Anti-TL1A antibody [EPR23330-2] (ab270456)

TL1A was immunoprecipitated from 0.35 mg HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate with ab270456 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270456 at 270456 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

Lane 1: HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate 10 ug

Lane 2: ab270456 IP in HT-29 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270456 in mouse ovary tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 32 seconds
Western blot - Anti-TL1A antibody [EPR23330-2] (ab270456)

All lanes : Anti-TL1A antibody [EPR23330-2] (ab270456) at 1/1000 dilution

Lane 1 : HUVEC (human umbilical vein endothelial cell), whole cell lysate at 20 µg
Lane 2 : HUVEC treated with/ml TNF-alpha for 24 hours, whole cell lysate 20

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 28 kDa
Observed band size: 25-36 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 59 seconds.

TL1A is a glycoprotein, the molecular weight observed is consistent with what have been described in literature (PMID 20675618).

The expression of TL1A is induced by TNFa in HUVEC (PMID: 15847792).
Anti-TL1A antibody [EPR23330-2] (ab270456)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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