Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)
![Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)](https://www.abcam.com/ps/products/229/ab229444/Images/ab229444-295376-anti-fadd-antibody-epr5030-immunohistochemistry.gif "Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5030] to FADD - BSA and Azide free
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse
Overview
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Product name
Anti-FADD antibody [EPR5030] - BSA and Azide free
See all FADD primary antibodies -
Description
Rabbit monoclonal [EPR5030] to FADD - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Mouse kidney tissue; Mouse spleen lysate, Raw264.7 cell lysate and NIH/3T3 cell lysate. Flow Cyt: NIH/3T3 cells
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General notes
ab229444 is the carrier-free version of ab124812 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab229444 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5030 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)
This IHC data was generated using the same anti-FADD antibody clone, EPR5030, in a different buffer formulation (cat# ab124812).
ab124812, at 1/50 dilution, staining FADD in paraffin-embedded Mouse Kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)](https://www.abcam.com/ps/products/229/ab229444/Images/ab229444-3-anti-fadd-epr5030-flowcytometry-nih-3t3-mouse.jpg)
Flow Cytometry - Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)This data was developed using ab124812, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling FADD with purified ab 124812 at 1/40 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue). -
![Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)](https://www.abcam.com/ps/products/229/ab229444/Images/ab229444-2-benefits-of-recombinant-antibodies.png)
Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)
