Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)
![Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)](https://www.abcam.com/ps/products/68/ab68475/Images/ab68475-82769-anti-fadd-phospho-s194-antibody-epr1816y-western-blot.jpg "Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1816Y] to FADD (phospho S194)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-FADD (phospho S194) antibody [EPR1816Y]
See all FADD primary antibodies -
Description
Rabbit monoclonal [EPR1816Y] to FADD (phospho S194) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human FADD aa 150-250 (phospho S194). The exact sequence is proprietary.
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Positive control
- Jurkat cell lysate
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1816Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)All lanes : Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475) at 1/500 dilution
Lane 1 : Un-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : Calyculin A treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
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![Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)](https://www.abcam.com/ps/products/68/ab68475/Images/ab68475-2-benefits-of-recombinant-antibodies.png)
Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)
