Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] - BSA and Azide free (ab256542)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22876-121] to KIR3DL1 + KIR3DS1 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR22876-121] to KIR3DL1 + KIR3DS1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NK-92 treated with 2µM 5-aza-2-deoxycytidine for 48 hours whole cell lysate, HEK-293T transfected with KIR3DL1 expression vector containing a myc-His-tag, whole cell lysate, HEK-293T transfected with KIR3DS1 expression vector containing a myc-His-tag, whole cell lysate; IHC-P: Human endometrium, KK-T lymphoma and spleen tissue.

  • General notes

    Ab256542 is the carrier-free version of ab222100. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256542 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] - BSA and Azide free (ab256542)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] - BSA and Azide free (ab256542)

    Immunohistochemical analysis of paraffin-embedded human NK-T lymphoma tissue labeling KIR3DL1+KIR3DS1 with ab222100 at 1/500 dilution (1.438 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human NK-T lymphoma (PMID: 22140359) is observed. The section was incubated with ab222100 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222100).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] - BSA and Azide free (ab256542)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] - BSA and Azide free (ab256542)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling KIR3DL1+KIR3DS1 with ab222100 at 1/500 dilution (1.438 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in some lymphocytes of human spleen (PMID: 22140359) is observed. The section was incubated with ab222100 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222100).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] - BSA and Azide free (ab256542)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] - BSA and Azide free (ab256542)

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR3DL1+KIR3DS1 with ab222100 at 1/500 dilution (1.438 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in some stromal cells of human endometrium (PMID: 22140359) is observed. The section was incubated with ab222100 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222100).

  • Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] - BSA and Azide free (ab256542)
    Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] - BSA and Azide free (ab256542)

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