Antibodies, Proteins, Kits and Reagents for Life Science

526 012 ₸ Product size

100 µg 526 012 ₸ 1 mg 4 690 ₸

Order now and get it on Tuesday March 09, 2021

Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] - BSA and Azide free (ab256542)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22876-121] to KIR3DL1 + KIR3DS1 - BSA and Azide free
Suitable for: IHC-P, WB
Reacts with: Human

Product name

Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] - BSA and Azide free
Description

Rabbit monoclonal [EPR22876-121] to KIR3DL1 + KIR3DS1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: NK-92 treated with 2µM 5-aza-2-deoxycytidine for 48 hours whole cell lysate, HEK-293T transfected with KIR3DL1 expression vector containing a myc-His-tag, whole cell lysate, HEK-293T transfected with KIR3DS1 expression vector containing a myc-His-tag, whole cell lysate; IHC-P: Human endometrium, KK-T lymphoma and spleen tissue.
General notes
Ab256542 is the carrier-free version of ab222100. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab256542 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22876-121
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] - BSA and Azide free (ab256542)

Immunohistochemical analysis of paraffin-embedded human NK-T lymphoma tissue labeling KIR3DL1+KIR3DS1 with ab222100 at 1/500 dilution (1.438 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human NK-T lymphoma (PMID: 22140359) is observed. The section was incubated with ab222100 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222100).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] - BSA and Azide free (ab256542)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling KIR3DL1+KIR3DS1 with ab222100 at 1/500 dilution (1.438 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in some lymphocytes of human spleen (PMID: 22140359) is observed. The section was incubated with ab222100 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222100).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] - BSA and Azide free (ab256542)

Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR3DL1+KIR3DS1 with ab222100 at 1/500 dilution (1.438 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in some stromal cells of human endometrium (PMID: 22140359) is observed. The section was incubated with ab222100 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222100).
Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] - BSA and Azide free (ab256542)

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