Anti-ALDH2 antibody (ab227021)
Key features and details
- Rabbit polyclonal to ALDH2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-ALDH2 antibody
See all ALDH2 primary antibodies -
Description
Rabbit polyclonal to ALDH2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig -
Immunogen
Recombinant fragment within Human ALDH2 (internal sequence). The exact sequence is proprietary.
Database link: P05091 -
Positive control
- ICC/IF: A549 cells. IHC-P: Mouse prostate and colon tissues; AGS xenograft tissue. WB: HepG2 whole cell and cytoplasmic extracts; GL261, C8D30, BCL-1 and RAW 264.7 whole cell lysates.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-ALDH2 antibody (ab227021) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cytoplasmic extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 56 kDa10% SDS-PAGE gel.
-
A549 (human lung carcinoma cell line) cells stained for ALDH2 (green) using ab227021 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin antibody diluted at 1/200 dilution.
Blue: Hoechst 33342 staining.
-
Paraffin-embedded mouse prostate tissue stained for ALDH2 using ab227021 at 1/500 dilution in immunohistochemical analysis.
-
Paraffin-embedded mouse colon tissue stained for ALDH2 using ab227021 at 1/500 dilution in immunohistochemical analysis.
-
All lanes : Anti-ALDH2 antibody (ab227021) at 1/1000 dilution
Lane 1 : GL261 whole cell lysate
Lane 2 : C8D30 whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 56 kDa7.5% SDS-PAGE gel.
-
Paraffin-embedded AGS xenograft tissue stained for ALDH2 using ab227021 at 1/100 dilution in immunohistochemical analysis.