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Anti-ALDH2 antibody (ab227021)

Price and availability

271 382 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-ALDH2 antibody (ab227021)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to ALDH2
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ALDH2 antibody
    See all ALDH2 primary antibodies
  • Description

    Rabbit polyclonal to ALDH2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Recombinant fragment within Human ALDH2 (internal sequence). The exact sequence is proprietary.
    Database link: P05091

  • Positive control

    • ICC/IF: A549 cells. IHC-P: Mouse prostate and colon tissues; AGS xenograft tissue. WB: HepG2 whole cell and cytoplasmic extracts; GL261, C8D30, BCL-1 and RAW 264.7 whole cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Alcohol metabolism
    • Cardiovascular
    • Atherosclerosis
    • Ischemia / Reperfusion
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Alcohol metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Heart disease

Images

  • Western blot - Anti-ALDH2 antibody (ab227021)
    Western blot - Anti-ALDH2 antibody (ab227021)
    All lanes : Anti-ALDH2 antibody (ab227021) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cytoplasmic extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 56 kDa



    10% SDS-PAGE gel.

  • Immunocytochemistry/ Immunofluorescence - Anti-ALDH2 antibody (ab227021)
    Immunocytochemistry/ Immunofluorescence - Anti-ALDH2 antibody (ab227021)

    A549 (human lung carcinoma cell line) cells stained for ALDH2 (green) using ab227021 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Red: phalloidin, a cytoskeleton marker, stained by phalloidin antibody diluted at 1/200 dilution.

    Blue: Hoechst 33342 staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody (ab227021)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody (ab227021)

    Paraffin-embedded mouse prostate tissue stained for ALDH2 using ab227021 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody (ab227021)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody (ab227021)

    Paraffin-embedded mouse colon tissue stained for ALDH2 using ab227021 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-ALDH2 antibody (ab227021)
    Western blot - Anti-ALDH2 antibody (ab227021)
    All lanes : Anti-ALDH2 antibody (ab227021) at 1/1000 dilution

    Lane 1 : GL261 whole cell lysate
    Lane 2 : C8D30 whole cell lysate
    Lane 3 : BCL-1 whole cell lysate
    Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 56 kDa



    7.5% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody (ab227021)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH2 antibody (ab227021)

    Paraffin-embedded AGS xenograft tissue stained for ALDH2 using ab227021 at 1/100 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-ALDH2 antibody (ab227021)

  •  
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    Anti-ALDH2 antibody [EPR4494] (ab133306)

    Applications: WB

  •  
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    Anti-ALDH2 antibody [9A10AF10] (ab110311)

    Applications: ICC/IF, IP

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