Anti-ATP5O antibody (ab230325)
Key features and details
- Rabbit polyclonal to ATP5O
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATP5O antibody
See all ATP5O primary antibodies -
Description
Rabbit polyclonal to ATP5O -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Pig, Orangutan
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Immunogen
Synthetic peptide corresponding to Human ATP5O aa 1-100.
Database link: P48047 -
Positive control
- IHC-P:Human skeletal muscle tissue. WB: HEK-293 cell lysate. Flow: HEK-293 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ATP5O antibody (ab230325)Anti-ATP5O antibody (ab230325) at 1/1000 dilution + HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 35 µg
Predicted band size: 23 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5O antibody (ab230325)Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for ATP50 using ab230325 (5 µg/ml) in immunohistochemical analysis.
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Flow Cytometry - Anti-ATP5O antibody (ab230325)Flow cytometric analysis of HEK-293 (human epithelial cell line from embryonic kidney) cells labeling ATP50 with ab230325 at 1/10 dilution (green) compared with a negative control (blue).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot - Anti-ATP5O antibody (ab230325)All lanes : Anti-ATP5O antibody (ab230325) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : ATP50-transfected HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lysates/proteins at 2 µg per lane.
Predicted band size: 23 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5O antibody (ab230325)Paraffin-embedded human skeletal muscle stained for ATP5O (green) using ab230325 (0.05 mg/ml) in immunohistochemical analysis.
FITC-conjugated goat anti-rabbit lgG was used as the secondary antibody. Counterstained with Propidium Iodide (red).
