Anti-GRAP2 antibody [OTI1G2] (ab119244)
![Anti-GRAP2 antibody [OTI1G2] (ab119244)](https://www.abcam.com/ps/products/119/ab119244/Images/ab119244-132146-anti-grap2-antibody-oti1g2-western-blot.jpg "Anti-GRAP2 antibody [OTI1G2] (ab119244)")
Key features and details
- Mouse monoclonal [OTI1G2] to GRAP2
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Dog, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-GRAP2 antibody [OTI1G2]
See all GRAP2 primary antibodies -
Description
Mouse monoclonal [OTI1G2] to GRAP2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human GRAP2 aa 1-350. Produced in HEK-293T cells (NP_004801).
Database link: O75791 -
Positive control
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY GRAP2 cDNA; HepG2 and Jurkat cell extracts. IHC-P: Human endometrium, endometrium adenocarcinoma and bladder carcinoma tissues. Flow Cyt: Jurkat cells and HEK-293T cells transfected with pCMV6-ENTRY GRAP2 cDNA.
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General notes
Clone OTI1G2 (formerly 1G2).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI1G2 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-GRAP2 antibody [OTI1G2] (ab119244)All lanes : Anti-GRAP2 antibody [OTI1G2] (ab119244) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY GRAP2 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 38 kDa
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![Western blot - Anti-GRAP2 antibody [OTI1G2] (ab119244)](https://www.abcam.com/ps/products/119/ab119244/Images/ab119244-132145-anti-grap2-antibody-oti1g2-western-blot.jpg)
Western blot - Anti-GRAP2 antibody [OTI1G2] (ab119244)All lanes : Anti-GRAP2 antibody [OTI1G2] (ab119244) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : SVT2 cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (Canine kidney cell line) cell extract
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 38 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAP2 antibody [OTI1G2] (ab119244)](https://www.abcam.com/ps/products/119/ab119244/Images/ab119244-132144-anti-grap2-antibody-oti1g2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAP2 antibody [OTI1G2] (ab119244)Paraffin-embedded human endometrium tissue stained for GRAP2 using ab119244 at 1/150 in mmunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAP2 antibody [OTI1G2] (ab119244)](https://www.abcam.com/ps/products/119/ab119244/Images/ab119244-132143-anti-grap2-antibody-oti1g2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAP2 antibody [OTI1G2] (ab119244)Paraffin-embedded human endometrium adenocarcinoma tissue stained for GRAP2 using ab119244 at 1/150 in mmunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAP2 antibody [OTI1G2] (ab119244)](https://www.abcam.com/ps/products/119/ab119244/Images/ab119244-132142-anti-grap2-antibody-oti1g2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAP2 antibody [OTI1G2] (ab119244)Paraffin-embedded human bladder carcinoma tissue stained for GRAP2 using ab119244 at 1/150 in mmunohistochemical analysis.
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![Flow Cytometry - Anti-GRAP2 antibody [OTI1G2] (ab119244)](https://www.abcam.com/ps/products/119/ab119244/Images/ab119244-132141-anti-grap2-antibody-oti1g2-flow-cytometry.jpg)
Flow Cytometry - Anti-GRAP2 antibody [OTI1G2] (ab119244)Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY GRAP2 overexpress plasmid (Red) or empty vector control plasmid (Blue) labeling GRAP2 using ab119244 at 1/100 dilution.
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![Flow Cytometry - Anti-GRAP2 antibody [OTI1G2] (ab119244)](https://www.abcam.com/ps/products/119/ab119244/Images/ab119244-132140-anti-grap2-antibody-oti1g2-flow-cytometry.jpg)
Flow Cytometry - Anti-GRAP2 antibody [OTI1G2] (ab119244)Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells stained for GRAP2 using ab119244 at 1/100 dilution (Red) compared to a nonspecific negative control antibody (Blue).
