Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)
![Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)](https://www.abcam.com/ps/products/183/ab183947/Images/ab183947-252067-anti-olfactory-marker-protein-antibody-epr19190-western-blot.jpg "Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19190] to Olfactory Marker Protein/OMP
- Suitable for: IHC-Fr, IHC-P, WB, IP
- Reacts with: Mouse, Rat
Overview
-
Product name
Anti-Olfactory Marker Protein/OMP antibody [EPR19190]
See all Olfactory Marker Protein/OMP primary antibodies -
Description
Rabbit monoclonal [EPR19190] to Olfactory Marker Protein/OMP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseRatIHC-P MouseIP MouseWB MouseRat
-
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Mouse and Rat olfactory bulb lysates. IHC-P: Mouse olfactory bulb tissue.
-
General notes
This product was previously labelled as Olfactory Marker Protein.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19190 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)All lanes : Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947) at 1/2000 dilution
Lane 1 : Mouse olfactory bulb lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse heart lysate
Lane 4 : Mouse kidney lysate
Lane 5 : Mouse spleen lysate
Lane 6 : Neuro-2a (Mouse neuroblastoma cell line) lysate
Lane 7 : Rat olfactory bulb lysate
Lane 8 : Rat kidney lysate
Lane 9 : C6 (Rat glial tumor cell line) lysate
Lane 10 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) lysate
Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma cell line) lysate
Lane 12 : NIH/3T3 (Mouse embryo fibroblast cell line) lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Immunoprecipitation - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)](https://www.abcam.com/ps/products/183/ab183947/Images/ab183947-252299-anti-olfactory-marker-protein-antibody-epr19190-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)Olfactory Marker Protein/OMP was immunoprecipitated from 1mg of Mouse olfactory bulb whole cell lysate with ab183947 at 1/40 dilution. Western blot was performed using ab183947 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Lane 1(input): Mouse olfactory bulb whole cell lysate 10μg
Lane 2: Mouse olfactory bulb whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab183947 in Mouse olfactory bulb whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)](https://www.abcam.com/ps/products/183/ab183947/Images/ab183947-252068-anti-olfactory-marker-protein-antibody-epr19190-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)Immunohistochemical analysis of paraffin-embedded Mouse olfactory bulb tissue labeling Olfactory Marker Protein/OMP with ab183947 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on neurons of mouse olfactory bulb is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)](https://www.abcam.com/ps/products/183/ab183947/Images/ab183947-252069-anti-olfactory-marker-protein-antibody-epr19190-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Olfactory Marker Protein/OMP with ab183947 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Negative staining on mouse liver is observed.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Frozen sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)](https://www.abcam.com/ps/products/183/ab183947/Images/ab183947-254623-anti-olfactory-marker-protein-antibody-epr19190-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)Immunohistochemistry (Frozen sections) analysis of mouse olfactory bulb tissue labelling Olfactory Marker Protein/OMP with ab183947 at a dilution of 1/500. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000). Staining is visible in the cytoplasm of neurons of the mouse olfactory bulb.
-
![Immunohistochemistry (Frozen sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)](https://www.abcam.com/ps/products/183/ab183947/Images/ab183947-254624-anti-olfactory-marker-protein-antibody-epr19190-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)Immunohistochemistry (Frozen sections) analysis of rat olfactory bulb tissue labelling Olfactory Marker Protein/OMP with ab183947 at a dilution of 1/500. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000). Staining is visible in the cytoplasm of neurons of the rat olfactory bulb.
-
![Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)](https://www.abcam.com/ps/products/183/ab183947/Images/ab183947-8-benefits-of-recombinant-antibodies.png)
Anti-Olfactory Marker Protein/OMP antibody [EPR19190] (ab183947)
