Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Thursday March 04, 2021

Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19157] to Olfactory Marker Protein/OMP
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Recombinant fragment

Product name

Anti-Olfactory Marker Protein/OMP antibody [EPR19157]
See all Olfactory Marker Protein/OMP primary antibodies
Description

Rabbit monoclonal [EPR19157] to Olfactory Marker Protein/OMP
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat
WB	Mouse Rat Recombinant fragment

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse OMP full length recombinant protein; Mouse and rat olfactory bulb lysates. IHC-P: Mouse and rat olfactory bulb tissues.
General notes
This product was previously labelled as Olfactory Marker Protein

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19157
Isotype

IgG
Research areas

Western blot - Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)

All lanes : Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948) at 1/1000 dilution

Lane 1 : Rat kidney lysate at 20 µg
Lane 2 : Rat spleen lysate at 20 µg
Lane 3 : Mouse brain lysate at 20 µg
Lane 4 : Mouse heart lysate at 20 µg
Lane 5 : Mouse kidney lysate at 20 µg
Lane 6 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg
Lane 7 : Mouse OMP full length recombinant protein at 0.02 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 19 kDa
Observed band size: 19 kDa

Exposure time: 3 minutes

Blocking/dilution buffer: 5% NFDM/TBST.
Uniquely associated with mature olfactory receptor neurons.
Mouse OMP full length recombinant protein contains aa 2-163 with a His-Tag^®. The recombinant mouse full length protein was made in-house.
Western blot - Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)

Lane 1 : Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948) at 1/200 dilution
Lane 2 : Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948) at 1/1000 dilution

Lane 1 : Mouse olfactory bulb lysate
Lane 2 : Rat olfactory bulb lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 19 kDa
Observed band size: 19 kDa

Exposure time: 3 minutes

Blocking/dilution buffer: 5% NFDM/TBST.
Uniquely associated with mature olfactory receptor neurons.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)

Immunohistochemical analysis of paraffin-embedded mouse olfactory bulb tissue labeling Olfactory Marker Protein/OMP with ab183948 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on neurons of mouse olfactory bulb is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Olfactory Marker Protein/OMP with ab183948 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on mouse liver. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)

Immunohistochemical analysis of paraffin-embedded rat olfactory bulb tissue labeling Olfactory Marker Protein/OMP with ab183948 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on neurons of rat olfactory bulb is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)

Immunohistochemical analysis of paraffin-embedded rat muscle tissue labeling Olfactory Marker Protein/OMP with ab183948 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on rat muscle. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Olfactory Marker Protein/OMP antibody [EPR19157] (ab183948)

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