HRP Anti-EGFR antibody [EP38Y] (ab193602)
![HRP Anti-EGFR antibody [EP38Y] (ab193602)](https://www.abcam.com/ps/products/193/ab193602/Images/ab193602-232895-anti-egfr-antibody-ep38y-hrp-immunohistochemistry.jpg "HRP Anti-EGFR antibody [EP38Y] (ab193602)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP38Y] to EGFR
- Suitable for: IHC-P, WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-EGFR antibody [EP38Y]
See all EGFR primary antibodies -
Description
HRP Rabbit monoclonal [EP38Y] to EGFR -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa whole cell lysate (ab150035). IHC: Normal human placenta tissue
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Dissociation constant (KD)
KD = 1.90 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EP38Y -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-EGFR antibody [EP38Y] (ab193602)
IHC image of EGFR staining in a section of formalin-fixed paraffin-embedded normal human placenta tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30 mins. The section was incubated with ab193602 at a working dilution of 1 in 500 overnight at +4°C. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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![Western blot - HRP Anti-EGFR antibody [EP38Y] (ab193602)](https://www.abcam.com/ps/products/193/ab193602/Images/ab193602-232692-anti-egfr-antibody-ep38y-hrp-western-blot.jpg)
Western blot - HRP Anti-EGFR antibody [EP38Y] (ab193602)HRP Anti-EGFR antibody [EP38Y] (ab193602) at 1/5000 dilution +HeLa whole cell lysate (ab150035) at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 134 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab193602 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-EGFR antibody [EP38Y] (ab193602)](https://www.abcam.com/ps/products/193/ab193602/Images/ab193602-3-benefits-of-recombinant-antibodies.png)
HRP Anti-EGFR antibody [EP38Y] (ab193602)
