Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)
![Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)](https://www.abcam.com/ps/products/227/ab227737/Images/ab227737-338479-anti-egfr-deleted-e746-l747-r748-e749-a750-antibody-sp111-immunofluorescence.jpg "Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP111] to EGFR (deleted E746 + L747 + R748 + E749 + A750)
- Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111]
See all EGFR primary antibodies -
Description
Rabbit monoclonal [SP111] to EGFR (deleted E746 + L747 + R748 + E749 + A750) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human lung carcinoma tissue. WB: HCC827 cell lysate. ICC/IF: HCC827 cells Flow Cyt: HCC827 cells
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General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP111 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)Immunocytochemistry/ Immunofluorescence analysis of HCC827(human lung adenocarcinoma epithelial cell), negative control:
A431(human epidermoid carcinoma epithelial cell) cells labeling EGFR with purified ab227737 at 1:100 (4.1 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Flow Cytometry - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)](https://www.abcam.com/ps/products/227/ab227737/Images/ab227737-338499-anti-egfr-deleted-e746-l747-r748-e749-a750-antibody-sp111-flow-cytometry.jpg)
Flow Cytometry - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)Flow Cytometry analysis of HCC827(Human lung adenocarcinoma epithelial cell) cells labeling EGFR with purified ab227737 at 1:400 dilution (1.02 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)](https://www.abcam.com/ps/products/227/ab227737/Images/ab227737-301577-anti-egfr-deleted-e746-l747-r748-e749-a750-antibody-sp111-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for EGFR (deleted E746 + L747 + R748 + E749 + A750) using ab227737 at 1/100 dilution in immunohistochemical analysis.
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![Western blot - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)](https://www.abcam.com/ps/products/227/ab227737/Images/ab227737-301576-anti-egfr-deleted-e746-l747-r748-e749-a750-antibody-sp111-western-blot.jpg)
Western blot - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737) at 1/300 dilution + HCC827 cell lysate
Predicted band size: 134 kDa
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![Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)](https://www.abcam.com/ps/products/227/ab227737/Images/ab227737-5-benefits-of-recombinant-antibodies.png)
Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)
