Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP111] to EGFR (deleted E746 + L747 + R748 + E749 + A750)
  • Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111]
    See all EGFR primary antibodies

  • Description

    Rabbit monoclonal [SP111] to EGFR (deleted E746 + L747 + R748 + E749 + A750)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human lung carcinoma tissue. WB: HCC827 cell lysate. ICC/IF: HCC827 cells Flow Cyt: HCC827 cells

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)
    Immunocytochemistry/ Immunofluorescence - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)
    Immunocytochemistry/ Immunofluorescence analysis of HCC827(human lung adenocarcinoma epithelial cell), negative control:
    A431(human epidermoid carcinoma epithelial cell) cells labeling EGFR with purified ab227737 at 1:100 (4.1 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)
    Flow Cytometry - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)
    Flow Cytometry analysis of HCC827(Human lung adenocarcinoma epithelial cell) cells labeling EGFR with purified ab227737 at 1:400 dilution (1.02 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)

    Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for EGFR (deleted E746 + L747 + R748 + E749 + A750) using ab227737 at 1/100 dilution in immunohistochemical analysis.

  • Western blot - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)
    Western blot - Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)
    Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737) at 1/300 dilution + HCC827 cell lysate

    Predicted band size: 134 kDa

  • Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)
    Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] (ab227737)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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