Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EGFR (phospho T654) antibody [EP2255Y] (ab75986)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2255Y] to EGFR (phospho T654)
  • Suitable for: WB, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-EGFR (phospho T654) antibody [EP2255Y]
    See all EGFR primary antibodies

  • Description

    Rabbit monoclonal [EP2255Y] to EGFR (phospho T654)

  • Host species

    Rabbit

  • Specificity

    Recognises EGFR phosphorylated on Threonine 654 of the mature human isoform 1 (corresponding to T678 from the precursor form P00533-1/p170)

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    corresponding to Human EGFR (phospho T654).

  • Positive control

    • WB: EGF treated A431 whole cell lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-EGFR (phospho T654) antibody [EP2255Y] (ab75986)
    Western blot - Anti-EGFR (phospho T654) antibody [EP2255Y] (ab75986)
    All lanes : Anti-EGFR (phospho T654) antibody [EP2255Y] (ab75986) at 1/1000 dilution (Purified)

    Lane 1 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
    Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) treated with 100ng/ml EGF for 30 minutes whole cell lysate
    Lane 3 : A431 (Human epidermoid carcinoma epithelial cell) treated with 100ng/ml EGF for 30 minutes whole cell lysate, then the membrane treated with Lambda Phosphotase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 134 kDa
    Observed band size: 170 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

  • Dot Blot - Anti-EGFR (phospho T654) antibody [EP2255Y] (ab75986)
    Dot Blot - Anti-EGFR (phospho T654) antibody [EP2255Y] (ab75986)

    Primary antibody dilution: 1/1000

    Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

    Secondary antibody dilution: 1/2500

    Blocking & dilution buffer: 5% NFDM/TBST

    Lane 1 sample: EGFR (pT654) phospho peptide

    Lane 2 sample: EGFR non-phospho peptide

    Exposure time: 3 minutes

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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