Anti-EGFR (phospho T654) antibody [EP2255Y] (ab75986)
![Anti-EGFR (phospho T654) antibody [EP2255Y] (ab75986)](https://www.abcam.com/ps/products/75/ab75986/Images/ab75986-450784-anti-egfr-phospho-t654-antibody-ep2255y-westernblot-a431-treated-with-egf-human.jpg "Anti-EGFR (phospho T654) antibody [EP2255Y] (ab75986)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2255Y] to EGFR (phospho T654)
- Suitable for: WB, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-EGFR (phospho T654) antibody [EP2255Y]
See all EGFR primary antibodies -
Description
Rabbit monoclonal [EP2255Y] to EGFR (phospho T654) -
Host species
Rabbit -
Specificity
Recognises EGFR phosphorylated on Threonine 654 of the mature human isoform 1 (corresponding to T678 from the precursor form P00533-1/p170) -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
corresponding to Human EGFR (phospho T654).
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Positive control
- WB: EGF treated A431 whole cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2255Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EGFR (phospho T654) antibody [EP2255Y] (ab75986)All lanes : Anti-EGFR (phospho T654) antibody [EP2255Y] (ab75986) at 1/1000 dilution (Purified)
Lane 1 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) treated with 100ng/ml EGF for 30 minutes whole cell lysate
Lane 3 : A431 (Human epidermoid carcinoma epithelial cell) treated with 100ng/ml EGF for 30 minutes whole cell lysate, then the membrane treated with Lambda Phosphotase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 134 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
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![Dot Blot - Anti-EGFR (phospho T654) antibody [EP2255Y] (ab75986)](https://www.abcam.com/ps/products/75/ab75986/Images/ab75986-256543-anti-egfr-phospho-t654-antibody-ep2255y-dot-blot.jpg)
Dot Blot - Anti-EGFR (phospho T654) antibody [EP2255Y] (ab75986)Primary antibody dilution: 1/1000
Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated
Secondary antibody dilution: 1/2500
Blocking & dilution buffer: 5% NFDM/TBST
Lane 1 sample: EGFR (pT654) phospho peptide
Lane 2 sample: EGFR non-phospho peptide
Exposure time: 3 minutes
