Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling EGFR with ab227647 at 1/100 dilution (2.67 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human lung carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227647 for 10 mins at room temperature.

Flow cytometry analysis of H1975 (Human non-small cell lung cancer epithelial cell) labeling EGFR with purified ab227647 at 1/30 dilution (8.9 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (black). Unlableled control -Unlabelled cells (blue).

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for EGFR (mutated L858R) using ab227647 at 1/100 dilution in immunohistochemical analysis.