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Signal Transduction Protein Phosphorylation Tyrosine Kinases Receptor Tyrosine Kinases

Anti-EGFR (phospho S671) antibody [EPR2257] (ab92629)

Price and availability

251 280 ₸

Availability

Order now and get it on Friday March 12, 2021

Anti-EGFR (phospho S671) antibody [EPR2257] (ab92629)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2257] to EGFR (phospho S671)
  • Suitable for: WB, ICC/IF, Dot blot
  • Reacts with: Human

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Overview

  • Product name

    Anti-EGFR (phospho S671) antibody [EPR2257]
    See all EGFR primary antibodies
  • Description

    Rabbit monoclonal [EPR2257] to EGFR (phospho S671)
  • Host species

    Rabbit
  • Specificity

    Recognises EGFR phosphorylated on serine 671 of the mature human isoform 1 (corresponding to S695 from the precursor form P00533-1/p170)
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    corresponding to Human EGFR (phospho S671).

  • Positive control

    • A431 cell lysate treated with EGF
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2257
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • EGF
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Cancer
    • Growth factors
    • EGF
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Tyrosine kinases
    • Receptor tyrosine kinases
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors
    • Cancer
    • Tumor biomarkers
    • Receptors

Images

  • Western blot - Anti-EGFR (phospho S671) antibody [EPR2257] (ab92629)
    Western blot - Anti-EGFR (phospho S671) antibody [EPR2257] (ab92629)
    All lanes : Anti-EGFR (phospho S671) antibody [EPR2257] (ab92629) at 1/1000 dilution

    Lane 1 : A431 cell lysate untreated
    Lane 2 : A431 cell lysate treated with EGF

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 134 kDa

  • Dot Blot - Anti-EGFR (phospho S671) antibody [EPR2257] (ab92629)
    Dot Blot - Anti-EGFR (phospho S671) antibody [EPR2257] (ab92629)

    Primary antibody dilution: 1/1000

    Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

    Secondary antibody dilution: 1/2500

    Blocking & dilution buffer: 5% NFDM/TBST

    Lane 1 sample: EGFR (pS671) phospho peptide

    Lane 2 sample: EGFR non-phospho peptide

    Exposure time: 3 minutes

  • Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S671) antibody [EPR2257] (ab92629)
    Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S671) antibody [EPR2257] (ab92629)
    Immunofluorescent staining of A431 cells using ab92629 at 1/100 dilution.
  • Anti-EGFR (phospho S671) antibody [EPR2257] (ab92629)
    Anti-EGFR (phospho S671) antibody [EPR2257] (ab92629)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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