Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478)
![Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478)](https://www.abcam.com/ps/products/68/ab68478/Images/ab68478-82774-anti-erbb-4-phospho-y1162-antibody-ep2270y-western-blot.jpg "Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2270Y] to ErbB4 / HER4 (phospho Y1162)
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y]
See all ErbB4 / HER4 primary antibodies -
Description
Rabbit monoclonal [EP2270Y] to ErbB4 / HER4 (phospho Y1162) -
Host species
Rabbit -
Specificity
This antibody detectsErbB4 / HER4 phosphorylated on tyrosine 1162.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
corresponding to Human ErbB4/ HER4. A phospho peptide corresponding to residues surrounding tyrosine 1162 of human ErbB4/ HER4.
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Positive control
- A431 cell lysate, human breast carcinoma tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2270Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478)All lanes : Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478) at 1/500 dilution
Lane 1 : A431 cell lysate. Cells untreated.
Lane 2 : A431 cell lysate. Cells treated with EGF.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 147 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478)](https://www.abcam.com/ps/products/68/ab68478/Images/ab68478-82773-anti-erbb-4-phospho-y1162-antibody-ep2270y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478)Human breast carcinoma stained with ab68478 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478)](https://www.abcam.com/ps/products/68/ab68478/Images/ab68478-3-benefits-of-recombinant-antibodies.png)
Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478)
