Anti-GAS 6 antibody [EPR23931-27] (ab264098)
![Anti-GAS 6 antibody [EPR23931-27] (ab264098)](https://www.abcam.com/ps/products/264/ab264098/Images/ab264098-1-anti-gas-6-antibody-epr2393127-flow-cytometry-thp1.jpg "Anti-GAS 6 antibody [EPR23931-27] (ab264098)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23931-27] to GAS 6
- Suitable for: Flow Cyt (Intra), IP, WB
- Reacts with: Human
Overview
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Product name
Anti-GAS 6 antibody [EPR23931-27]
See all GAS 6 primary antibodies -
Description
Rabbit monoclonal [EPR23931-27] to GAS 6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: THP-1, SH-SY5Y and RPMI-8226 whole cell lysates Flow Cyt: THP-1 and THP-1 cells. IP: THP-1 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23931-27 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-GAS 6 antibody [EPR23931-27] (ab264098)
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized THP-1 (Human monocytic leukemia monocyte) cells labelling GAS 6 with ab264098 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab264098.
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![Western blot - Anti-GAS 6 antibody [EPR23931-27] (ab264098)](https://www.abcam.com/ps/products/264/ab264098/Images/ab264098-454970-anti-gas-6-antibody-epr2393127-western-blot-leukemia.jpg)
Western blot - Anti-GAS 6 antibody [EPR23931-27] (ab264098)All lanes : Anti-GAS 6 antibody [EPR23931-27] (ab264098) at 1/1000 dilution
Lane 1 : THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 2 : Jurkat (human t cell leukemia T lymphocyte) whole cell lysate
Lane 3 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 4 : RPMI-8226 (human plasmacytoma, myeloma B lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 75 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: Jurkat (PMID:10400186)
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: Lanes 1-2: 48 seconds;Lanes 3-4: 3 minutes.
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![Flow Cytometry - Anti-GAS 6 antibody [EPR23931-27] (ab264098)](https://www.abcam.com/ps/products/264/ab264098/Images/ab264098-2-anti-gas-6-antibody-epr2393127-flow-cytometry-thp11.jpg)
Flow Cytometry - Anti-GAS 6 antibody [EPR23931-27] (ab264098)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) cells labelling GAS 6 with ab264098 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab264098. Cells were co-stained with DRAQ/ Ki-67 to differentiate cell cycle phase.
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![Immunoprecipitation - Anti-GAS 6 antibody [EPR23931-27] (ab264098)](https://www.abcam.com/ps/products/264/ab264098/Images/ab264098-4-anti-gas-6-antibody-epr2393127-immunoprecipitation-thp1.jpg)
Immunoprecipitation - Anti-GAS 6 antibody [EPR23931-27] (ab264098)GAS 6 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) whole cell lysate with ab264098 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab264098 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: THP-1 (human monocytic leukemia monocyte) whole cell lysate 10 ug
Lane 2: ab264098 IP in THP-1 whole cell lysate
Lane 3: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated: Rabbit monoclonal IgG (ab172730) instead of ab264098 in THP-1 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds
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![Flow Cytometry - Anti-GAS 6 antibody [EPR23931-27] (ab264098)](https://www.abcam.com/ps/products/264/ab264098/Images/ab264098-3-anti-gas-6-antibody-epr2393127-flow-cytometry-jurkat.jpg)
Flow Cytometry - Anti-GAS 6 antibody [EPR23931-27] (ab264098)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Jurkat (Human T cell leukemia T lymphocyte (Left) / THP-1 (Human monocytic leukemia monocyte, Right) cells labelling GAS 6 with ab264098 at 1/500 dilution (Right). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: Jurkat (PMID:10400186)
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![Anti-GAS 6 antibody [EPR23931-27] (ab264098)](https://www.abcam.com/ps/products/264/ab264098/Images/ab264098-455168-benefits-of-recombinant-antibodies.jpg)
Anti-GAS 6 antibody [EPR23931-27] (ab264098)