Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸ 10 µl 117 264 ₸

Order now and get it on Tuesday March 16, 2021

Anti-GAS 6 antibody [EPR23931-27] (ab264098)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23931-27] to GAS 6
Suitable for: Flow Cyt (Intra), IP, WB
Reacts with: Human

Product name

Anti-GAS 6 antibody [EPR23931-27]
See all GAS 6 primary antibodies
Description

Rabbit monoclonal [EPR23931-27] to GAS 6
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: THP-1, SH-SY5Y and RPMI-8226 whole cell lysates Flow Cyt: THP-1 and THP-1 cells. IP: THP-1 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23931-27
Isotype

IgG
Research areas

Flow Cytometry - Anti-GAS 6 antibody [EPR23931-27] (ab264098)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized THP-1 (Human monocytic leukemia monocyte) cells labelling GAS 6 with ab264098 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab264098.
Western blot - Anti-GAS 6 antibody [EPR23931-27] (ab264098)

All lanes : Anti-GAS 6 antibody [EPR23931-27] (ab264098) at 1/1000 dilution

Lane 1 : THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 2 : Jurkat (human t cell leukemia T lymphocyte) whole cell lysate
Lane 3 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 4 : RPMI-8226 (human plasmacytoma, myeloma B lymphocyte) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 75 kDa
Observed band size: 78 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Negative control: Jurkat (PMID:10400186)

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time: Lanes 1-2: 48 seconds;Lanes 3-4: 3 minutes.
Flow Cytometry - Anti-GAS 6 antibody [EPR23931-27] (ab264098)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) cells labelling GAS 6 with ab264098 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab264098. Cells were co-stained with DRAQ/ Ki-67 to differentiate cell cycle phase.
Immunoprecipitation - Anti-GAS 6 antibody [EPR23931-27] (ab264098)

GAS 6 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) whole cell lysate with ab264098 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab264098 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: THP-1 (human monocytic leukemia monocyte) whole cell lysate 10 ug

Lane 2: ab264098 IP in THP-1 whole cell lysate

Lane 3: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated: Rabbit monoclonal IgG (ab172730) instead of ab264098 in THP-1 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 15 seconds
Flow Cytometry - Anti-GAS 6 antibody [EPR23931-27] (ab264098)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Jurkat (Human T cell leukemia T lymphocyte (Left) / THP-1 (Human monocytic leukemia monocyte, Right) cells labelling GAS 6 with ab264098 at 1/500 dilution (Right). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: Jurkat (PMID:10400186)
Anti-GAS 6 antibody [EPR23931-27] (ab264098)

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