Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-Calpain 2 antibody [EPR2562Y] (ab75994)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2562Y] to Calpain 2
Suitable for: WB, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-Calpain 2 antibody [EPR2562Y]
See all Calpain 2 primary antibodies
Description

Rabbit monoclonal [EPR2562Y] to Calpain 2
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Calpain 2 aa 150-250. The exact sequence is proprietary.
Positive control
- WB: HEK-293T, A431 and LNCaP cell lysates; HeLa cytoplasmic or membrane lysate. Flow Cyt: Permeabilized HeLa cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR2562Y
Isotype

IgG
Research areas

Western blot - Anti-Calpain 2 antibody [EPR2562Y] (ab75994)

All lanes : Anti-Calpain 2 antibody [EPR2562Y] (ab75994) at 1/2000 dilution

Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : CAPN2 knockout HEK-293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : LNCaP cell lysate

Lysates/proteins at 40 µg per lane.

Performed under reducing conditions.

Observed band size: 75 kDa
why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab75994 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab75994 was shown to react with Calpain 2 in wild-type HEK-293T cells in Western blot with loss of signal observed in CAPN2 knockout cell line ab266628 (CAPN2 knockout cell lysate ab257379). Wild-type HEK-293T and CAPN2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab75994 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Flow Cytometry - Anti-Calpain 2 antibody [EPR2562Y] (ab75994)

Flow cytometric analysis of permeabilized HeLa cells using ab75994 (red) or a rabbit IgG (negative) (green).
Western blot - Anti-Calpain 2 antibody [EPR2562Y] (ab75994)

Anti-Calpain 2 antibody [EPR2562Y] (ab75994) at 1/500 dilution + HeLa cell lysate at 10 µg

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Observed band size: 75 kDa why is the actual band size different from the predicted?
Anti-Calpain 2 antibody [EPR2562Y] (ab75994)

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