Anti-Calpain 2 antibody [EPR5977] (ab126600)
![Anti-Calpain 2 antibody [EPR5977] (ab126600)](https://www.abcam.com/ps/products/126/ab126600/Images/ab126600-393582-anti-calpain-2-antibody-epr5977-western-blot-human-knockout-lysate.jpg "Anti-Calpain 2 antibody [EPR5977] (ab126600)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5977] to Calpain 2
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Calpain 2 antibody [EPR5977]
See all Calpain 2 primary antibodies -
Description
Rabbit monoclonal [EPR5977] to Calpain 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB RatHuman
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Immunogen
Synthetic peptide within Human Calpain 2 aa 650-750. The exact sequence is proprietary.
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Positive control
- WB: PC3, HeLa, A431, LNCaP and 293T cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5977 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Calpain 2 antibody [EPR5977] (ab126600)All lanes : Anti-Calpain 2 antibody [EPR5977] (ab126600) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : CAPN2 knockout HEK293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : LNCaP cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDaLanes 1-4: Merged signal (red and green). Green - ab126600 observed at 80 kDa. Red - loading control ab8245 observed at 36 kDa.
ab126600 Anti-Calpain 2 antibody [EPR5977] was shown to specifically react with Calpain 2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266628 (knockout cell lysate ab257379) was used. Wild-type and Calpain 2 knockout samples were subjected to SDS-PAGE. ab126600 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Calpain 2 antibody [EPR5977] (ab126600)](https://www.abcam.com/ps/products/126/ab126600/Images/ab126600-307080-anti-calpain-2-antibody-epr5977-western-blot.jpg)
Western blot - Anti-Calpain 2 antibody [EPR5977] (ab126600)All lanes : Anti-Calpain 2 antibody [EPR5977] (ab126600) at 1/1000 dilution (Purified)
Lane 1 : Rat brain lysates
Lane 2 : Rat kidney lysates
Lane 3 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : Mouse brain lysates
Lane 5 : Mouse kidney lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 80 kDa
Observed band size: 80 kDa
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![Western blot - Anti-Calpain 2 antibody [EPR5977] (ab126600)](https://www.abcam.com/ps/products/126/ab126600/Images/ab126600-140560-anti-calpain-2-antibody-epr5977-western-blot.jpg)
Western blot - Anti-Calpain 2 antibody [EPR5977] (ab126600)All lanes : Anti-Calpain 2 antibody [EPR5977] (ab126600) at 1/1000 dilution (unpurified)
Lane 1 : PC3 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDa
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![Anti-Calpain 2 antibody [EPR5977] (ab126600)](https://www.abcam.com/ps/products/126/ab126600/Images/ab126600-4-benefits-of-recombinant-antibodies.png)
Anti-Calpain 2 antibody [EPR5977] (ab126600)
