Antibodies, Proteins, Kits and Reagents for Life Science

268 032 ₸ Product size

100 µl 268 032 ₸

Order now and get it on Wednesday March 10, 2021

Anti-ARFGAP1 antibody [EPR13650] (ab183746)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13650] to ARFGAP1
Suitable for: WB, ICC/IF
Reacts with: Human

Product name

Anti-ARFGAP1 antibody [EPR13650]
See all ARFGAP1 primary antibodies
Description

Rabbit monoclonal [EPR13650] to ARFGAP1
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal brain lysate; HepG2, HeLa and MCF7 cell lysates; HeLa and MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13650
Isotype

IgG
Research areas

Western blot - Anti-ARFGAP1 antibody [EPR13650] (ab183746)

All lanes : Anti-ARFGAP1 antibody [EPR13650] (ab183746) at 1/5000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

Predicted band size: 45 kDa
Observed band size: 45 kDa
Western blot - Anti-ARFGAP1 antibody [EPR13650] (ab183746)

All lanes : Anti-ARFGAP1 antibody [EPR13650] (ab183746) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

Predicted band size: 45 kDa
Observed band size: 45 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ARFGAP1 antibody [EPR13650] (ab183746)

Immunofluorescent analysis of acetone-fixed HeLa cells labeling ARFGAP1 with ab183746 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution (red). Counter stained with Dapi (blue).
Immunocytochemistry/ Immunofluorescence - Anti-ARFGAP1 antibody [EPR13650] (ab183746)

Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling ARFGAP1 with ab183746 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution (red). Counter stained with Dapi (blue).
Anti-ARFGAP1 antibody [EPR13650] (ab183746)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com