Anti-ARFGAP1 antibody [EPR13650] (ab183746)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13650] to ARFGAP1
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-ARFGAP1 antibody [EPR13650]
See all ARFGAP1 primary antibodies -
Description
Rabbit monoclonal [EPR13650] to ARFGAP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain lysate; HepG2, HeLa and MCF7 cell lysates; HeLa and MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13650 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ARFGAP1 antibody [EPR13650] (ab183746) at 1/5000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
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All lanes : Anti-ARFGAP1 antibody [EPR13650] (ab183746) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
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Immunofluorescent analysis of acetone-fixed HeLa cells labeling ARFGAP1 with ab183746 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution (red). Counter stained with Dapi (blue).
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Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling ARFGAP1 with ab183746 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution (red). Counter stained with Dapi (blue).
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