Anti-ARFGAP1 antibody (ab204405)
Key features and details
- Rabbit polyclonal to ARFGAP1
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ARFGAP1 antibody
See all ARFGAP1 primary antibodies -
Description
Rabbit polyclonal to ARFGAP1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to Human ARFGAP1 aa 100-250.
Database link: Q8N6T3 -
Positive control
- IHC-P: Human pancreas, placenta, and tonsil tissues; ICC: CACO-2 cells; WB: RT4 and U-251 MG cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGAP1 antibody (ab204405)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling ARFGAP1 with ab204405 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry - Anti-ARFGAP1 antibody (ab204405)Immunofluorescent analysis of CACO-2 cells labeling ARFGAP1 with ab204405 at 4 µg/mL. Fixation/Permeabilization: PFA/Triton X-100.
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Western blot - Anti-ARFGAP1 antibody (ab204405)All lanes : Anti-ARFGAP1 antibody (ab204405) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate
Predicted band size: 45 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGAP1 antibody (ab204405)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling ARFGAP1 with ab204405 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGAP1 antibody (ab204405)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling ARFGAP1 with ab204405 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGAP1 antibody (ab204405)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling ARFGAP1 with ab204405 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
