Anti-ARFGAP1 antibody [1C4E2] (ab234087)
Key features and details
- Mouse monoclonal [1C4E2] to ARFGAP1
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-ARFGAP1 antibody [1C4E2]
See all ARFGAP1 primary antibodies -
Description
Mouse monoclonal [1C4E2] to ARFGAP1 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human ARFGAP1 aa 250 to the C-terminus. Expressed in E. coli.
Database link: Q8N6T3-2 -
Positive control
- WB: MOLT-4, C2C12, HepG2, MCF7 and LNcap cell lysate; ARFGAP1 (amino acids: 270-414)-hIgGFc transfected HEK-293 cell lysate; Recombinant human ARFGAP1 (amino acids: 270-414) protein. Flow Cytometry: HepG2 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1C4E2 -
Isotype
IgG2b -
Research areas
Images
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All lanes : Anti-ARFGAP1 antibody [1C4E2] (ab234087) at 1/100 dilution
Lane 1 : MOLT-4 (human lymphoblastic leukemia cell line) cell lysate
Lane 2 : C2C12 (mouse myoblast cell line) cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 5 : LNCap (human prostate cancer cell line) cell lysate
Predicted band size: 45 kDa
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Flow Cytometry analysis of HepG2 (human liver hepatocellular carcinoma cell line) cells, labeling ARFGAP1 with ab234087 at 1/200 dilution (green) compared to a negative control (red).
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All lanes : Anti-ARFGAP1 antibody [1C4E2] (ab234087) at 1/100 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : ARFGAP1 (amino acids: 270-414)-hIgGFc transfected HEK-293 cell lysate
Predicted band size: 45 kDa
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Anti-ARFGAP1 antibody [1C4E2] (ab234087) at 1/100 dilution + Recombinant human ARFGAP1 (amino acids: 270-414) protein
Predicted band size: 45 kDaExpected recombinant MW is 41.5 kDa