Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Signal Transduction Protein Trafficking Vesicle Transport Regulation

Anti-ARFGAP1 antibody [1C4E2] (ab234087)

Anti-ARFGAP1 antibody [1C4E2] (ab234087)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [1C4E2] to ARFGAP1
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG2b

You may also be interested in

Product image
Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] (ab81551)
Product image
Anti-SF21 antibody - N-terminal (ab206685)
Product image
Anti-EEF2K antibody [EP881Y] (ab45168)
Product image
Anti-EIF2B2 antibody (ab204179)

Overview

  • Product name

    Anti-ARFGAP1 antibody [1C4E2]
    See all ARFGAP1 primary antibodies
  • Description

    Mouse monoclonal [1C4E2] to ARFGAP1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human, Recombinant fragment
  • Immunogen

    Recombinant fragment corresponding to Human ARFGAP1 aa 250 to the C-terminus. Expressed in E. coli.
    Database link: Q8N6T3-2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: MOLT-4, C2C12, HepG2, MCF7 and LNcap cell lysate; ARFGAP1 (amino acids: 270-414)-hIgGFc transfected HEK-293 cell lysate; Recombinant human ARFGAP1 (amino acids: 270-414) protein. Flow Cytometry: HepG2 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    1C4E2
  • Isotype

    IgG2b
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Signal Transduction
    • Protein Trafficking
    • ER Proteins
    • Signal Transduction
    • Protein Trafficking
    • Golgi Proteins

Images

  • Western blot - Anti-ARFGAP1 antibody [1C4E2] (ab234087)
    Western blot - Anti-ARFGAP1 antibody [1C4E2] (ab234087)
    All lanes : Anti-ARFGAP1 antibody [1C4E2] (ab234087) at 1/100 dilution

    Lane 1 : MOLT-4 (human lymphoblastic leukemia cell line) cell lysate
    Lane 2 : C2C12 (mouse myoblast cell line) cell lysate
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma cell line) cell lysate
    Lane 5 : LNCap (human prostate cancer cell line) cell lysate

    Predicted band size: 45 kDa

  • Flow Cytometry - Anti-ARFGAP1 antibody [1C4E2] (ab234087)
    Flow Cytometry - Anti-ARFGAP1 antibody [1C4E2] (ab234087)

    Flow Cytometry analysis of HepG2 (human liver hepatocellular carcinoma cell line) cells, labeling ARFGAP1 with ab234087 at 1/200 dilution (green) compared to a negative control (red).

  • Western blot - Anti-ARFGAP1 antibody [1C4E2] (ab234087)
    Western blot - Anti-ARFGAP1 antibody [1C4E2] (ab234087)
    All lanes : Anti-ARFGAP1 antibody [1C4E2] (ab234087) at 1/100 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : ARFGAP1 (amino acids: 270-414)-hIgGFc transfected HEK-293 cell lysate

    Predicted band size: 45 kDa

  • Western blot - Anti-ARFGAP1 antibody [1C4E2] (ab234087)
    Western blot - Anti-ARFGAP1 antibody [1C4E2] (ab234087)
    Anti-ARFGAP1 antibody [1C4E2] (ab234087) at 1/100 dilution + Recombinant human ARFGAP1 (amino acids: 270-414) protein

    Predicted band size: 45 kDa



    Expected recombinant MW is 41.5 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-ARFGAP1 antibody [1C4E2] (ab234087)

  •  
  • Product image

    Anti-ARFGAP1 antibody (ab204405)

    Applications: ICC, IHC-P, WB

  •  
  • Product image

    Anti-ARFGAP1 antibody [EPR13650] (ab183746)

    Applications: ICC/IF, WB

Clear all

Recently viewed products

  •  
  • Product image

    Anti-Cortactin antibody [4F11] (ab33333)

  •  
  • Product image

    Anti-ENPP1/PC1 antibody [EPR22262-22] (ab223268)

  •  
  • Product image

    Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)

  •  
  • Product image

    Anti-RING1 antibody [8C12F4] (ab175149)

  •  
  • Product image

    Anti-Cdc25C antibody [E303] (ab32050)

  •  
  • Product image

    Anti-ERK2 antibody [1B3B9] (ab231085)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.