Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] - BSA and Azide free (ab247973)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5671] to PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] - BSA and Azide free
    See all PRK3+PRK2+PRK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5671] to PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects PKN3 phosphorylated at Threonine 718, PKN2 phosphorylated at Threonine 816, and PKN1 phosphorylated at Threonine 774.

  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247973 is the carrier-free version of ab124709. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247973 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] - BSA and Azide free (ab247973)
    Western blot - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] - BSA and Azide free (ab247973)
    All lanes : Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] (ab124709) at 1/1000 dilution

    Lane 1 : HeLa cell lysate untreated
    Lane 2 : HeLa cell lysate treated with Okadaic acid and Calyculin A.

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 104, 112, 99 kDa



    This data was developed using ab124709, the same antibody clone in a different buffer formulation.

    Bottom blot was probed with an anti-PNK2 antibody.

  • Flow Cytometry - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] - BSA and Azide free (ab247973)
    Flow Cytometry - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] - BSA and Azide free (ab247973)
    This data was developed using ab124709, the same antibody clone in a different buffer formulation.ab124709, at 1/10, staining PKN1 (phospho T774) + PKN2 (phospho T816) + PKN3 (phospho T718) in permeabilized Hek 293 cells by Flow cytometry.
    Green: untreated cells
    Red: Okadaic Acid + Calyculin A-treated cells
    Blue: untreated cells preincubated with the immunizing phosphopeptides
    Orange: Okadaic Acid + Calyculin A-treated cells preincubated with the immunizing phosphopeptides .
  • Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] - BSA and Azide free (ab247973)
    Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] - BSA and Azide free (ab247973)

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