Anti-TOM1 antibody [EPR11988(B)] (ab170866)
![Anti-TOM1 antibody [EPR11988(B)] (ab170866)](https://www.abcam.com/ps/products/170/ab170866/Images/ab170866-365778-anti-tom1-antibody-epr11988b-western-blot-knockout-hap1-tom1-hepg2-hela-human-lysates.jpg "Anti-TOM1 antibody [EPR11988(B)] (ab170866)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11988(B)] to TOM1
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-TOM1 antibody [EPR11988(B)]
See all TOM1 primary antibodies -
Description
Rabbit monoclonal [EPR11988(B)] to TOM1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide within Human TOM1 (Cysteine residue). The exact sequence is proprietary.
Database link: O60784 -
Positive control
- HepG2, JAR, HeLa and K562 cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11988(B) -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-TOM1 antibody [EPR11988(B)] (ab170866)All lanes : Anti-TOM1 antibody [EPR11988(B)] (ab170866) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : TOM1 knockout HAP1 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 54 kDaLanes 1 - 4: Merged signal (red and green). Green - ab170866 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab170866 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in TOM1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TOM1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab170866 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-TOM1 antibody [EPR11988(B)] (ab170866)](https://www.abcam.com/ps/products/170/ab170866/Images/ab170866-170750-ab1708661.jpg)
Western blot - Anti-TOM1 antibody [EPR11988(B)] (ab170866)All lanes : Anti-TOM1 antibody [EPR11988(B)] (ab170866) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : JAR cell lysates
Lane 3 : HeLa cell lysates
Lane 4 : K562 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa
-
![Anti-TOM1 antibody [EPR11988(B)] (ab170866)](https://www.abcam.com/ps/products/170/ab170866/Images/ab170866-3-benefits-of-recombinant-antibodies.png)
Anti-TOM1 antibody [EPR11988(B)] (ab170866)
