Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TOM1 antibody [EPR11988(B)] (ab170866)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11988(B)] to TOM1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-TOM1 antibody [EPR11988(B)]
    See all TOM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11988(B)] to TOM1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human TOM1 (Cysteine residue). The exact sequence is proprietary.
    Database link: O60784

  • Positive control

    • HepG2, JAR, HeLa and K562 cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11988(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TOM1 antibody [EPR11988(B)] (ab170866)
    Western blot - Anti-TOM1 antibody [EPR11988(B)] (ab170866)
    All lanes : Anti-TOM1 antibody [EPR11988(B)] (ab170866) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : TOM1 knockout HAP1 whole cell lysate
    Lane 3 : HepG2 whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 54 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab170866 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab170866 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in TOM1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TOM1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab170866 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-TOM1 antibody [EPR11988(B)] (ab170866)
    Western blot - Anti-TOM1 antibody [EPR11988(B)] (ab170866)
    All lanes : Anti-TOM1 antibody [EPR11988(B)] (ab170866) at 1/1000 dilution

    Lane 1 : HepG2 cell lysates
    Lane 2 : JAR cell lysates
    Lane 3 : HeLa cell lysates
    Lane 4 : K562 cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 54 kDa

  • Anti-TOM1 antibody [EPR11988(B)] (ab170866)
    Anti-TOM1 antibody [EPR11988(B)] (ab170866)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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