Anti-Fascin antibody (ab183891)
Key features and details
- Rabbit polyclonal to Fascin
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Fascin antibody
See all Fascin primary antibodies -
Description
Rabbit polyclonal to Fascin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human Fascin aa 89-365. The exact sequence is proprietary.
Database link: Q16658 -
Positive control
- Mouse ovary tissue, HCT116 cells, HeLa whole cell lysate/extract
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Fascin antibody (ab183891)All lanes : Anti-Fascin antibody (ab183891) at 1/2000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Fascin Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 54 kDa
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Western blot - Anti-Fascin antibody (ab183891)Anti-Fascin antibody (ab183891) at 1/1000 dilution + HeLa whole cell lysate/extract at 30 µg
Predicted band size: 54 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Fascin antibody (ab183891)Immunofluorescence analysis of HCT116 cells fixed in 100% methanol for 5 minutes, labeling Fascin using ab183891 at a 1/500 dilution (green). Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody (ab183891)Immunohistochemical analysis of paraffin-embedded mouse ovary tissue, labeling Fascin using ab183891 at a 1/500 dilution.
