Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Fascin antibody [FSCN1/417] - BSA and Azide free (ab212840)

Key features and details

Mouse monoclonal [FSCN1/417] to Fascin - BSA and Azide free
Suitable for: WB, ICC, Flow Cyt, IHC-P
Reacts with: Human
Isotype: IgG2a

Product name

Anti-Fascin antibody [FSCN1/417] - BSA and Azide free
See all Fascin primary antibodies
Description

Mouse monoclonal [FSCN1/417] to Fascin - BSA and Azide free
Host species

Mouse
Tested applications

Suitable for: WB, ICC, Flow Cyt, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant full length protein corresponding to Human Fascin aa 1 to the C-terminus.
Database link: Q16658

Run BLAST with Run BLAST with
Positive control
- IHC-P: Human Hodgkin's lymphoma, ovarian carcinoma and testicular carcinoma tissues; WB: HeLa cell lysate; ICC: HeLa cells; Flow Cyt: K562 cells.
General notes

ab212840 is a carrier free version of ab220195. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

ab22195 was purified from Bioreactor Concentrate by Protein A/G.
Clonality

Monoclonal
Clone number

FSCN1/417
Isotype

IgG2a
Light chain type

kappa
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody (ab212840)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human Hodgkin's lymphoma tissue labeling Fascin with ab220195 at 1 μg/ml dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220195).
Immunocytochemistry - Anti-Fascin antibody [FSCN1/417] - BSA and Azide free (ab212840)

Immunofluorescence Analysis of HeLa cells labeling Fascin with ab212840 (Green). The nuclear counterstain is Hoescht (Blue). Membrane is Phalloidin 488 (Red) .
Flow Cytometry - Anti-Fascin antibody [FSCN1/417] - BSA and Azide free (ab212840)

Flow Cytometric Analysis of PFA-fixed K562 cells labeling Fascin-1 with ab212840 followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)
Western blot - Anti-Fascin antibody [FSCN1/417] - BSA and Azide free (ab212840)

Anti-Fascin antibody [FSCN1/417] - BSA and Azide free (ab212840) at 2 µg/ml + HeLa cell lysate

Predicted band size: 54 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody (ab212840)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human ovarian carcinoma tissue labeling Fascin with ab220195 at 1 μg/ml dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220195).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody (ab212840)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human testicular carcinoma tissue labeling Fascin with ab220195 at 1 μg/ml dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220195).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com