Anti-Fascin antibody (ab97753)
Key features and details
- Rabbit polyclonal to Fascin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Fascin antibody
See all Fascin primary antibodies -
Description
Rabbit polyclonal to Fascin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant protein fragment corresponding to a region within amino acids 162-356 of Human Fascin (NP_003079)
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Positive control
- HeLa and HepG2 whole cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Fascin antibody (ab97753)All lanes : Anti-Fascin antibody (ab97753) at 1/500 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Fascin Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 55 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
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Western blot - Anti-Fascin antibody (ab97753)All lanes : Anti-Fascin antibody (ab97753) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 55 kDa
7.5% SDS PAGE
