Recombinant Human IL-2RG protein (Fc Chimera) (ab84065)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
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Product name
Recombinant Human IL-2RG protein (Fc Chimera)
See all IL-2RG proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
Theoretical Sequence: LNTTILTPNGNEDTTADFFLTTMPTDSLSVSTLPLPEVQ CFVFNVEYM NCTWNSSSEPQPTNLTLHYWYKNSDNDKVQKCSHYLFSE EITSGCQLQ KKEIHLYQTFVVQLQDPREPRRQATQMLKLQNLVIPWAP ENLTLHKLS ESQLELNWNNRFLNHCLEHLVQYRTDWDHSWTEQSVDYR HKFSLPSVD GQKRYTFRVRSRFNPLCGSAQHWSEWSHPIHWGSNTSKE NPFLFAWIP KVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPK DTLMISRTP EVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQY NSTYRVVSV LTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPR EPQVYTLPP SRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYK TTPPVLDSD GSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKS LSLSPGK -
Amino acids
23 to 259 -
Additional sequence information
DNA sequence encoding the signal peptide and extracellular domain of human IL 2R gamma (aa 1-259) was fused to the Fc region of human IgG1 (aa 93-330). Protein expressed in modified human 293 cells.
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Preparation and Storage
- CD132
- CD132 antigen
- CIDX
Images
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SDS-PAGE - Recombinant Human IL-2RG protein (Fc Chimera) (ab84065)1D SDS-PAGE of ab84065 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab84065
Lane 2: ab84065 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab84065 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
10 μg protein loaded per lane; Deep Purple™ stained. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Subsequent drop in MW after treatment with glycosidase cocktail indicates presence of O-linked glycans. Faint bands in lane 2 and lane 3 are glycosidase enzymes. -
SDS-PAGE - Recombinant Human IL-2RG protein (Fc Chimera) (ab84065)A sample of ab84065 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel.
40 μg protein loaded per lane; Deep Purple™ stained. Spot train indicates presence of multiple isoforms. Spots within the spot train were cut from the gel and identified as IL-2RG (Fc Chimera) by protein mass fingerprinting. -
Functional Studies - Recombinant Human IL-2RG protein (Fc Chimera) (ab84065)Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MW and pI of the protein.
