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Granzyme B Antibody Panel (EPR8260, EPR20129-217, BLR022E) (ab252202)

Price and availability

348 441 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Granzyme B Antibody Panel (EPR8260, EPR20129-217, BLR022E) (ab252202)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

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Overview

  • Product name

    Granzyme B Antibody Panel (EPR8260, EPR20129-217, BLR022E)
    See all Granzyme B kits
  • Species reactivity

    Reacts with: Human
  • Product overview

    Granzyme B Antibody Panel ab252202 contains multiple trial-sized versions of anti-human antibody clones against Granzyme B, specifically selected for high performance in IHC. This panel contains 3 recombinant rabbit monoclonal antibodies against human Granzyme B. They are provided in trial sizes to allow you to easily evaluate which is the best Granzyme B antibody for your IHC research assay needs.


    For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.


    Panel contains:


    - Rabbit monoclonal [BLR022E] to Granzyme B (20 µL) ab243879


    - Rabbit monoclonal [EPR20129-217] to Granzyme B (20 µL) ab208586


    - Rabbit monoclonal [EPR8260] to Granzyme B (20 µL) ab134933

  • Notes

    Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

    Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass CytometryTM. Please refer to the ‘Associated products’ section below.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 kit
    ab243879 - Anti-Granzyme B antibody [BLR022E] 2 x 10µl
    ab208586 - Anti-Granzyme B antibody [EPR20129-217] 2 x 10µl
    ab134933 - Anti-Granzyme B antibody [EPR8260] 2 x 10µl
  • Research areas

    • Cell Biology
    • Apoptosis
    • Extracellular Signals
    • Granzymes
    • Immunology
    • Adaptive Immunity
    • T Cells
    • Cytotoxic Cells
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Immunology
    • Innate Immunity
    • NK Cells
  • Function

    This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
  • Sequence similarities

    Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization

    Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
  • Target information above from: UniProt accession P10144 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • C11
    • Cathepsin G like 1
    • Cathepsin G-like 1
    • CCPI
    • CGL 1
    • CGL1
    • CSP B
    • CSPB
    • CTLA 1
    • CTLA-1
    • CTLA1
    • CTSGL1
    • Cytotoxic serine protease B
    • Cytotoxic T lymphocyte associated serine esterase 1
    • Cytotoxic T lymphocyte proteinase 2
    • Cytotoxic T-lymphocyte proteinase 2
    • Fragmentin 2
    • Fragmentin-2
    • GRAB_HUMAN
    • Granzyme 2
    • Granzyme B
    • Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1)
    • Granzyme-2
    • GranzymeB
    • GRB
    • Gzmb
    • Hlp
    • Human lymphocyte protein
    • Lymphocyte protease
    • Protease, serine, B
    • SECT
    • T cell serine protease 1 3E
    • T cell serine protease 1-3E
    • T-cell serine protease 1-3E
    see all
  • Database links

    • Entrez Gene: 3002 Human
    • Omim: 123910 Human
    • SwissProt: P10144 Human
    • Unigene: 1051 Human

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B [EPR8260]
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B [EPR8260]

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Granzyme B with Purified ab134933 at 1:250 dilution (2.96 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B [EPR20129-217]
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B [EPR20129-217]

      Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue labeling Granzyme B with ab208586 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on neutrophils and stroma cells of human cervix cancer is observed [PMID: 14512315]. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B [BLR022E]
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B [BLR022E]

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for Ki67 using ab243879 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

    • Granzyme B Antibody Panel (EPR8260, EPR20129-217, BLR022E) (ab252202)
      Granzyme B Antibody Panel (EPR8260, EPR20129-217, BLR022E) (ab252202)

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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